Introduction
The inflammation of hepatitis is swelling that occurs when tissues of the body are infected or injured. Hepatitis is characterised as acute or chronic, depending on how long the inflammation lasts and how much the hepatic parenchyma is damaged [1]. Viruses are classified into five types: A, B, C, D, and E. Because of the burden of illness and mortality, these five types are the most concerning. The disorder might be self-limiting (i.e., it will go away on its own) or it can develop to fibrosis (scarring) and cirrhosis [2]. Parasites and viral infections, autoimmune illnesses, and intoxication with xenobiotics such as alcohol, herbal medicine, pharmaceuticals, chlorinated solvents, peroxidized fatty acids, fungal toxins, industrial pollutants, and radioactive isotopes are all examples of hepatic problems.Types A and C, in particular, cause chronic disease in hundreds of millions of people and are the leading causes of liver cirrhosis and cancer [3]. In India, medicinal herbs are a key source of hepatoprotective drugs. More than 700 mono and polyherbal preparations in the form of decoctions, tinctures, and pills have been used for various liver ailments [4]. Ayurveda is the earliest Indian system of medicine, defining many plants for the management of hepatotoxicity. Since times plants have been used therapeutically in variety of conditions and by the arrival of current synthetic medicines and their accessibility of consistent dosage forms, usage ease and theatrical efficiency in acute circumstances, but there was decrease in the usage of medicinal plant till herbal rebellion [5].  As compared to the narrow spectrum effect of synthetic drugs with the associated danger of side effects, traditional drugs show minor action and require long term administration to be effective, mainly in chronic conditions [6]. Many plant-based medications, such as Andrographis paniculata, Boerrhavia diffusa, Calotropis procera,Fumaria indica, Garcinia cambogia, Luffa acutangula, Mamordic subangulata, Naragamia alata, Nigella sativa, and Trigonella foenum graecum have been proven to be hepatoprotective in nature. [7]. Hepatitis and jaundice are major liver ailments, with an elevated toll of mortality. At present, only rare hepatoprotective allopathic medicines are accessible for the management of liver ailments. As a result, people are adapting various plant extracts for the treatment of liver syndromes. From both a morphological and functional standpoint, the galactosamine-induced experimental model system in rats is acknowledged to be similar to viral hepatitis in humans. Galactosamine has a high liver specificity compared to other dangerous groups such as acetaminophen, carbon tetrachloride, paracetamol and so on, because hepatocytes have high levels of galactose-1-uridyltransferase, galactokinase and galactosamine does not harm other organs. Hepatotocity is caused by galactosamine, which causes patchy hepatocyte necrosis and significant portal and parenchymal infiltration (4). By boosting the formation of UDP-sugar derivates, galactosamine causes uridine diphosphate (UDP) depletion, which causes inhibition of RNA and protein synthesis, leading to cell membrane deterioration [8]. This study was aimed at investigating the pretreatment of HepG2 cells with hepatoprotective agents against the damage induced by galactosamine (GalN). Hypertension is a serious medical chronic illness that gradually increases the risk of kidney, heart, brain, and other illnesses. Hypertension is a major cause of death that takes place before the average age of death in a certain population worldwide. Hypertension affects an estimated 1.28 billion people globally, the majority of whom live in middle and low-income nations. It increases the risk of cardiovascular diseases, including diffuse atherosclerosis, heart failure, dementia, and cognitive impairment. Synthetically, antihypertensive drugs have been suggested to treat hypertension. However, the therapeutic activity of these drugs is low and also generates various side effects, which involve visual disorders, dry mouth, dizziness, emotional distress, gastrointestinal disturbances, etc. Therefore, it requires searching for cheaper and non- toxic natural products. Plants are generally used in the traditional system of medicine for the treatment of several diseases. In the past few decades, a lot of investigation has been done on local medicinal plants for hypotensive and antihypertensive potential. Plants are a rich source of flavonoid glycosides, which may have antihypertensive potential. The present investigation is focused on the effect of the anti-hypertensive activity of flavonoids and glycosides on rat induced methylprednisolne hypertension. Boerhavia diffusa is a herbaceous Nyctaginaceae family member with a long history of use by Brazil’s indigenous and tribal peoples. In particular, the roots and leaves of this plant have been widely used in folk medicine to treat several illnesses, including those affecting the gastrointestinal tract. This plant has been reported for behavioural and neuroendocrine effects [1], antidepressant activity [2], anti-angiogenic activity [3], anticonvulsant and antiepileptic [4], anti-inflammatory activity [5], prostatic hyperplasia [6], genotoxic and antigenotoxic [7], thrombolytic, cytotoxic and antimicrobial activities [8]. arsenic trioxide induced cardiotoxicity [9], antiurolithic activity [10], anti-hyperglycaemic and reno-protective effect [11], acetaminophen induced liver toxicity studies [12] , antiproliferative and antiestrogenic effects [13],  immunomodulatory, anti-metastatic activity [15] , radio-protective activity [16], potent anti-breast cancer activity [17], intestinal activity [18], immunosuppressive activity [19], anti-diabetic activity [20],   CA2+ channel antagonistic [21],  chemo-preventive action [22], glycol-induced urolithiasis [23], and inhibition of  human cervical cancer cells [24].  Several flavonoid glycosides are isolated from the leaves of this plant that show potent anticancer, powerful antioxidant and geno-protective properties, intestinal motility, and spasmolytic activity [25]. The objective of the study was to optimize, validate and quantify eupalitin 3-O- β-D-galactopyranoside in hydroalcoholic extracts of B. diffusa and also investigate hepatoprotective and antihypertensive activity of isolated eupalitin 3-O- β-D-galactopyranoside.