Isolation of eupalitin 3-O- β-D-galactopyranoside
Dried powdered plant material was subjected to extraction by using
methanol for 6 h at 37°C respectively. The combined hydro alcoholic
extracts were pooled and evaporated to dryness under reduced pressure at
40 ºC with rotator evaporator. Extracts was filtered and concentrated in
rotary evaporator under reduced pressure to get residues (28%). The
crude extracts obtained were portioned-fractionated with hexane (3×250
ml), chloroform (3×250 ml) and ethyl acetate (3×250 ml). The dried ethyl
acetate recidue (15 g) was thoroughly mixed in hot methanol and stored
overnight in the refrigerator, filtered, the solid seperated,
cristallized with hot methanol, to obtain eupalitin 3-O-
β-D-galactopyranoside (0.1578%w/w).