Effect of eupalitin- 3-O- β-D-galactopyranoside on galactosamine induced hepatotoxicity
In the study, we examined the possible hepatoprotective activity of purified eupalitin, remain ethyl acetate fraction and chloroform fraction against GalN-induced cytotoxicity, by pre-incubating the cells with or without the extracts or sylimarin. A significant decrease in cell viability was observed upon treatment of HepG2 cells with GalN (4Mm).Treatment with purified eupalitin and other fractions demonstrated a significant dose-depended protection toward cell toxicity resulting from GalN exposure. Results are expressed as mean ± standard error mean (S.E.M). From graph plotting, percentage protection against sample concentration, a sample concentration providing percentage cytoprotection of standard silymarin (84 percent at 500g/ml), purified eupalitin (67 percent at 1000g/ml), chloroform fraction (23 percent at 1000g/ml) and remain ethyl acetate fraction (42 percent at 500g/ml) was calculated. On Fig .3 and Table 5, the % protection is graphically represented. The experiment was carried out three times. Graphpad prism 5.0 was used for statistical analysis, which included Dunnett’s multiple comparison tests and one-way analysis of variance (ANOVA) (Graphpad Software, Inc., San Diego, CA, USA).