Instruments
Plant material extracts were created by hot percolation using Soxhlet extractor (Omega, Mumbai, India). Extracts were dried underneath vacuum on Rotary evaporator (Buchi R-114, Switzerland). For HPTLC analysis of bioactive compounds, a cAMAG HPTLC system (Muttenz, Switzerland) equipped with a Linomat IV sample applicator was used. Extracts were applied on aluminum- backed TLC plates (10 × 20 cm) pre-coated with silica gel 60F254 (Merck, Darmstadt, Germany). For developing the TLC plates, a CAMAG twin trough chamber (vertical development) was used. Tissue culture flasks, 96 and 24 well micro culture plates, eppendorf tube, inverted microscope, serological pipette, heamocytometer (Himedia Lab Pvt. Ltd., Mumbai, India),Laminar flow hoods (Khera instrument, New Delhi, India), CO2 incubator (NuAire, USA), Water bath, Deep freezer (-20°C).