Effect of eupalitin- 3-O- β-D-galactopyranoside on galactosamine
induced hepatotoxicity
In the study, we examined the possible hepatoprotective activity of
purified eupalitin, remain ethyl acetate fraction and chloroform
fraction against GalN-induced cytotoxicity, by pre-incubating the cells
with or without the extracts or sylimarin. A significant decrease in
cell viability was observed upon treatment of HepG2 cells with GalN
(4Mm).Treatment with purified eupalitin and other fractions demonstrated
a significant dose-depended protection toward cell toxicity resulting
from GalN exposure. Results are expressed as mean ± standard error mean
(S.E.M). From graph plotting, percentage protection against sample
concentration, a sample concentration providing percentage
cytoprotection of standard silymarin (84 percent at 500g/ml), purified
eupalitin (67 percent at 1000g/ml), chloroform fraction (23 percent at
1000g/ml) and remain ethyl acetate fraction (42 percent at 500g/ml) was
calculated. On Fig .3 and Table 5, the % protection is graphically
represented. The experiment was carried out three times. Graphpad prism
5.0 was used for statistical analysis, which included Dunnett’s multiple
comparison tests and one-way analysis of variance (ANOVA) (Graphpad
Software, Inc., San Diego, CA, USA).