Calibration curves for standard markers
Standard solution of Eupalitin 3-O- β-D-galactopyranoside was applied in
triplicate silica gel 60 F254 plates, using a CAMMAG
Linomat-5 Automatic Sample Spotter. The plates were developed in solvent
toluene: acetone: water (5: 15:1 v/v) in a CAMAG glass twin trough
chamber (20×10 cm) up to a space of 8 cm. After development, the plates
were dried in air and scanned at 254 nm using CAMAG TLC scanner 3 and
win CATS 4 software. The peak areas were recorded. Calibration curves of
Eupalitin 3-O- β-D-galactopyranoside were obtained by plotting peak
areas versus applied crude extracts concentration of Eupalitin 3-O-
β-D-galactopyranoside respectively.