Estimation of different markers
With a CAMAG Linomat-5 Automatic Sample Spotter, ten microliters of each
of the sample solutions were applied in triplicate on silica gel 60 F254
plates. The peak areas and absorption spectra were recorded. The number
of bioactive compounds in B. diffusa was calculated using the
respective standard calibration curves of Eupalitin 3-O-
β-D-galactopyranoside