Conclusion
Preparation of 80% methanolic extracts of Boerhavia diffusa by using Soxhlet extraction and fractionation of extracts based on the polarity. In the present study, Simultaneously determination of eupalitin O- β-D-galactopyranoside in Boerhavia diffusa leaves is carried out by HPTLC in solvent system of An efficient HPTLC method for method of validation and quantification of eupalitin-O- β-D-galactopyranoside in above solvent have been developed firstly. Isolation and purification of eupalitin-O- β-D-galactopyranoside was done by sedimentation method and characterization of isolated compound was performed by Mass spectroscopy. In-vitro Hepatoprotective Activity of isolated eupalitin-O- β-D-galactopyranoside was checked in HPG2 cell line compared with silymarin by ANNOVA technique. In vivo antihypertensive activity of isolated eupalitin O- β-D-galactopyranoside was done in intoxicated rats. It is concluded that methyl-predenisolone induced arterial hypertension was accompanied by elevation in plasma rennin activity and administration of isolated eupalitin-O- β-D-galactopyranoside caused more significant drops in mean arterial blood pressure compared with toxic group by ANNOVA technique.
Acknowledgments: The authors acknowledge the financial support by National Medicinal Plant Board (NMPB), AYUSH Project No: R&D/P-01/2010-2011. Govt. of India. The authors extend their appreciation to the Deanship of scientific research at King Khalid University, Grant No: RGP2/ 191/42 Saudi Arabia for financial assistance.
The contribution of authors is as follows: Conceptualization, methodology, investigation, data curation, IUK; VA conceived the presented project idea, writing—original draft preparation, writing—review and editing, visualization, supervision, funding acquisition.
Data Availability Statement: The data that supports the finding of this study are also available from the corresponding author upon request.
Competing Interest Statement: The authors declare no conflict of interest.