Conclusion
Preparation of 80% methanolic extracts of Boerhavia diffusa by
using Soxhlet extraction and fractionation of extracts based on the
polarity. In the present study, Simultaneously determination of
eupalitin O- β-D-galactopyranoside in Boerhavia diffusa leaves is
carried out by HPTLC in solvent system of An efficient HPTLC method for
method of validation and quantification of eupalitin-O-
β-D-galactopyranoside in above solvent have been developed
firstly. Isolation and purification of eupalitin-O-
β-D-galactopyranoside was done by sedimentation method and
characterization of isolated compound was performed by Mass
spectroscopy. In-vitro Hepatoprotective Activity of isolated
eupalitin-O- β-D-galactopyranoside was checked in HPG2 cell line
compared with silymarin by ANNOVA technique. In vivo antihypertensive
activity of isolated eupalitin O- β-D-galactopyranoside was done in
intoxicated rats. It is concluded that methyl-predenisolone induced
arterial hypertension was accompanied by elevation in plasma rennin
activity and administration of isolated eupalitin-O-
β-D-galactopyranoside caused more significant drops in mean arterial
blood pressure compared with toxic group by ANNOVA technique.
Acknowledgments: The authors acknowledge the financial support
by National Medicinal Plant Board (NMPB), AYUSH Project No:
R&D/P-01/2010-2011. Govt. of India. The authors extend their
appreciation to the Deanship of scientific research at King Khalid
University, Grant No: RGP2/ 191/42 Saudi Arabia for financial
assistance.
The contribution of authors is as follows: Conceptualization,
methodology, investigation, data curation, IUK; VA conceived the
presented project idea, writing—original draft preparation,
writing—review and editing, visualization, supervision, funding
acquisition.
Data Availability Statement: The data that supports the finding
of this study are also available from the corresponding author upon
request.
Competing Interest Statement: The authors declare no conflict
of interest.