Estimation of different markers
With a CAMAG Linomat-5 Automatic Sample Spotter, ten microliters of each of the sample solutions were applied in triplicate on silica gel 60 F254 plates. The peak areas and absorption spectra were recorded. The number of bioactive compounds in B. diffusa was calculated using the respective standard calibration curves of Eupalitin 3-O- β-D-galactopyranoside