Effect of independent factors on Eupalitin 3-O- β-D-galactopyranoside (Y1)
Regression equation of the fitted models:Y1= 0.035-0.00097 X1 + 0.006 X2 + 0.0031 X3 + 0.0038 X1 X2 + 0.0043 X1 X3 - 0.0051 X2X3 The extraction time (X1) affected on the % yield of eupalitin. It has observed from the equation X1 have negative effect on eupalitin as compared to X2 and X3. When increase time of extraction increases, the % yield of eupalitin decreases due to degradation of compound as shown figure in (Fig. 2A). Factor X2 (temperature) have prominent positive effect on % yield of eupalitin than factor X1 and X3. As the temperature increased, the % yield of eupalitin also increased respectively due to thermo stable compound as shown in (Fig. 2B). Factor X3(solvent ratio) showed less positive effect on eupalitin percentage yield as compared to that observed on factor X2. It was observed that, when higher level of solvent ratio was increased, % yield of eupalitin also increased because medium polar compound as shown figure in (Fig. 2C).