Instruments
Plant material extracts were created by hot percolation using Soxhlet
extractor (Omega, Mumbai, India). Extracts were dried underneath vacuum
on Rotary evaporator (Buchi R-114, Switzerland). For HPTLC analysis of
bioactive compounds, a cAMAG HPTLC system (Muttenz, Switzerland)
equipped with a Linomat IV sample applicator was used. Extracts were
applied on aluminum- backed TLC plates (10 × 20 cm) pre-coated with
silica gel 60F254 (Merck, Darmstadt, Germany). For
developing the TLC plates, a CAMAG twin trough chamber (vertical
development) was used. Tissue culture flasks, 96 and 24 well micro
culture plates, eppendorf tube, inverted microscope, serological
pipette, heamocytometer (Himedia Lab Pvt. Ltd., Mumbai, India),Laminar flow hoods (Khera instrument, New Delhi, India),
CO2 incubator (NuAire, USA), Water bath, Deep freezer
(-20°C).