Optimal mobile phase development
The working standard solution of Eupalitin 3-O- β-D-galactopyranoside
(1m/ml) in methanol was used for chromatographic separation
investigations. Initially, multiple solvent solutions were used in
various trials. Finally: toluene: acetone: water (5: 15:1) for
determination of Eupalitin 3-O- β-D-galactopyranoside gave a sharp and
well-defined peak. We obtained well-defined bands upon saturating the
chamber with the mobile fraction at room temperature for 30 min. The
standard band of Eupalitin 3-O- β-D-galactopyranoside along with
hydroalcoholic extracts of B. diffusa presented on HPTLC plate
scanned at 366 nm (Fig. 1).