Maintenance of cell lines:
HepG2 cell line were grown in 25 cm2 tissue culture flasks containing minimum essential medium (MEM) supplemented with 10% FBS, 1% penicillin /streptomycin at 37°C in a CO2incubator in an atmosphere of humidified 5% CO2 and 95% air. The cells were kept alive by sub-culturing them in 25 cm2 tissue culture flasks on a regular basis.