Calibration curves for standard markers
Standard solution of Eupalitin 3-O- β-D-galactopyranoside was applied in triplicate silica gel 60 F254 plates, using a CAMMAG Linomat-5 Automatic Sample Spotter. The plates were developed in solvent toluene: acetone: water (5: 15:1 v/v) in a CAMAG glass twin trough chamber (20×10 cm) up to a space of 8 cm. After development, the plates were dried in air and scanned at 254 nm using CAMAG TLC scanner 3 and win CATS 4 software. The peak areas were recorded. Calibration curves of Eupalitin 3-O- β-D-galactopyranoside were obtained by plotting peak areas versus applied crude extracts concentration of Eupalitin 3-O- β-D-galactopyranoside respectively.