Isolation of eupalitin 3-O- β-D-galactopyranoside
Dried powdered plant material was subjected to extraction by using methanol for 6 h at 37°C respectively. The combined hydro alcoholic extracts were pooled and evaporated to dryness under reduced pressure at 40 ºC with rotator evaporator. Extracts was filtered and concentrated in rotary evaporator under reduced pressure to get residues (28%). The crude extracts obtained were portioned-fractionated with hexane (3×250 ml), chloroform (3×250 ml) and ethyl acetate (3×250 ml). The dried ethyl acetate recidue (15 g) was thoroughly mixed in hot methanol and stored overnight in the refrigerator, filtered, the solid seperated, cristallized with hot methanol, to obtain eupalitin 3-O- β-D-galactopyranoside (0.1578%w/w).