Chemicals and reagents
Isolated and purified of eupalitin 3-O- β-D-galactopyranoside from
Hamdard laboratory, New Delhi, India. Methanol HPLC grade (SD Fine
Chemicals, Mumbai, India) was used as a solvent for the preparation of
standards and samples. Toluene, ethyl acetate, formic acid, and methanol
(CDH Labs, Mumbai, India) were used as mobile phases for HPTLC analysis.
2- Amino ethyl diphenylborinate (Sigma-Aldrich Co. LLC, St. Louis, MO,
USA). All solutions used for the analysis were filtered through a 0.22
μm Syringe- driven Filter (HIMEDIA, Mumbai, India). Dulbecco’s modified
eagle’s medium (DMEM), foetal bovine serum (FBS), HepG2 cell line
(National Centre for Cell Science (NCCS), Pune, India), MTT test kit,
trypsin EDTA, Trypan blue solution, and 100% ethanol (Himedia Lab Pvt.
Ltd., Mumbai, India), Penicillin and Streptomycin, and DMSO
(Sigma-Aldrich Co. LLC, St. Louis, MO, USA).