Anti-sera
As described previously (Zhu et al., 2018), six three-week-old SPF
chickens were immunized twice by subcutaneous injection of 0.3 mL of
oil-emulsion of inactivated whole virus vaccines of the viruses F/98 and
rF/HAA198V, which were inactivated by adding 0.2%
formalin (v /v) for 24 h at 37 °C, respectively. The antisera were
collected and pooled from the vaccinated SPF chickens at three weeks
after the vaccination.