3.5 Oral administration of β-damascone ameliorates contact
hypersensitivity in mice
Finally, we investigated whether β-damascone, which inhibits DC-mediated
inflammation in in vitro experiments, exhibits a biologically
significant effect on immunorelated diseases by using CHS model mice
(Figure 5A ). First, we examined the effects of various doses of
β-damascone on ear swelling symptoms in DNFB-treated mice and found that
oral administration of β-damascone at 10 or 50 mg/kg per day from 2 days
before sensitization suppressed ear swelling (data not shown). Under
optimized doses, the suppressive effect of β-damascone on ear swelling
was observed not only after challenge but also after rechallenge
(Figure 5B ), accompanied by a tendency of reduced mRNA levels
of Il6 in the lesional skin (data not shown). These results
indicate that orally administered β-damascone modulates DC-induced
immunoresponses in vivo .