2.5 Quantitative RT-PCR
Total RNA was extracted from BMDCs using the ReliaPrep RNA Cell Miniprep System (#Z6012, Promega, Madison, USA) and from the skin using ISOGEN (#311-07361, Nippongene, Tokyo, Japan). Synthesis of cDNA, and quantitative PCR were performed as previously described10. The nucleotide sequences of the primer sets are listed in Supplementary Table SI .