ZDHHC9 structural connectome - Supplementary Materials
The structural connectome was based on streamline tractography. A connections between regions was indicated if at least X number of fibres intersected with both ROIs. The resulting connectome description depends on the ability of the tractography algorithm to provide a biologically meaningful account of macroscopic white matter connection. The results presented in the main manuscript are based on a constrained spherical deconvolution model with probabilistic tractography. This approach is able to resolve crossing fibres (Tournier 2008) and shows high overlap in comparison with post-mortem tract tracing (Calamante 2012). The following figure shows a representative example of the whole brain connectome constructed through this approach. Please note that the density is considerably reduced in this depiction; only every 20th streamline is shown.
Visualization of connectome results is associated with various limitations. There is a general trade-off between providing anatomical context and presenting a complete account of connections (Margulies 2013). The images presented in the main manuscript provide the anatomical context, but had to be thresholded at a high cut-off. Otherwise, numerous connections with low edge weight would have obscured the image. Here, unthresholded connection matrices are presented that show all connections, but do not provide anatomical context.