Tissue collection
Two ethmoid sinus tissue biopsies were collected by an Ear, Nose and
Throat surgeon at baseline and at weeks 4, 8, 16 and 24 following the
administration of local anaesthetic to each nostril (300µL, 5%
lignocaine hydrochloride/0.5% phenylephrine hydrochloride). One biopsy
was fixed in 10% neutral buffered formalin for histopathology and the
second snap-frozen in liquid nitrogen, and stored at
-170oC until further processing.