Figure 4. Trace data from PCR products by mixing powder of different proportions. A: P. ginseng and P. quinquefoliusin the ratio of 9:1. B: P. ginseng and P. quinquefolius in the ratio of 7:3. C: P. ginseng and P. quinquefolius in the ratio of 5:5. D: P. ginseng and P. quinquefolius in the ratio of 3:7. E: P. ginseng and P. quinquefolius in the ratio of 1:9.
Supplemental Information
Supplementary Figure 1. Taxonomic analysis of prescription ingredients in the four samples detected via reads belonging to the matK region. Each taxonomic node is drawn as a bar chart, indicating the number of reads assigned to the taxon for each sample.
Supplementary Figure 2. Taxonomic analysis of prescription ingredients in the four samples detected via reads belonging to the rbcL region. Each taxonomic node is drawn as a bar chart indicating the number of reads assigned to the taxon for each sample.
Supplementary Figure 3. Trace data from PCR products by four pharmaceutical samples.
Supplementary Figure 4. Distribution of the fungi for each sample at the genus level.
Supplementary Figure 5. Heat map of the fungi genus identified in six samples analysed by shotgun metabarcoding. Yellow and blue colors indicate present and absent fungi genus, respectively.
Supplementary Figure 6. Mapping results of matK region in the HSZY171 sample with the whole genome of A. macrocephala as the reference sequence. (a) is the whole chloroplast genome sequence ofA. macrocephala . (b) and (c) are the assembly sequences. (d) is the matK standard sequence of A. macrocephala .
Supplementary Table 1 Medicinal materials collected for made mock SLBZS samples.
Supplementary Table 2 Proportion of the five ingredients listed in the prescription of SLBZS.
Supplementary Table 3 Shotgun sequencing results of the two mock and four SLBZS pharmaceutical samples.
Supplementary Table 4. Reads number of the prescription ingredients in the four pharmaceutical samples based on the ITS2 sequences.
Supplementary Table 5. Reads number of the prescription ingredients in the four pharmaceutical samples based on thepsbA-trnH sequences.
Supplementary Table 6. Reads number of the prescription ingredients in the four pharmaceutical samples based on the matKsequences.
Supplementary Table 7. Reads number of the prescription ingredients in the four pharmaceutical samples based on the rbcLsequences.
Supplementary Table 8. Reads number of the fungi in the six samples based on the ITS2 sequences.
Supplementary Table 9. Proportion of P. ginseng reads at the SNP positions in ITS2 in four pharmaceutical samples.