2. Materials and methods
2.1. Sample collectionand preparation of lab-made
mock SLBZS samples
Ten herbal materials of SLBZS were purchased from a Beijing Tongrentang
drug store (Figure 1) . DNA barcoding techniques were used to
obtain ITS2, psbA-trnH , matK , and rbcL of the
herbal materials. These barcodes are used to verify the accuracy of the
original species of herbal materials on the one hand, and on the other
hand to verify the accuracy of subsequent sequencing assembly
(Supplementary Table 1 ). Subsequently, these authenticated
herbal materials were used to make mock samples according to the
preparation method under the ”Shenling Baizhu San” item of the Chinese
Pharmacopoeia (2020 edition). The proportion of each medicinal material
listed in the preparation is shown in Supplementary Table 2 .
Two mock samples were made and named HSZY159 and HSZY171, respectively.
To verify the sensitivity of the method, 6.4 mg powder of Panax
quinquefolius root was added to each 100 mg of the mock sample HSZY171
in the same proportion as the lowest proportion of the prescribed
ingredient in SLBZS. In addition, four pharmaceutical samples of SLBZS
with different production batch numbers were collected from different
pharmacy stores, and were marked as A03, HSZY152, HSZY191, and HSZY192.