2.7.2 Pathogenicity in mice
Groups of five 6-week-old female BALB/c mice (Yangzhou Experimental
Animal Center, Jiangsu, China) were anaesthetized with pentobarbital
sodium and intranasally (i.n.)
inoculated with
106.5 egg infectious dose (EID50) of
H7N9 AIV isolates in a volume of 50 μl. Body weight changes and
mortality of the infected mice were monitored daily for 14 days. Mice
were humanely euthanized if weight loss was over 25% of their initial
weight and scored as dead for humane reasons.