2.7.1 Pathogenicity in chickens
According to the recommendations of the World Organization for Animal
Health (OIE, 2018),
ten
6-week-old SPF chickens per group were intravenously inoculated with 100
μl tenfold diluted virus-containing allantoic fluids. All the birds were
observed for 10 days to determine the intravenous pathogenicity index
(IVPI).