Figure legends
Figure 1. Phylogenetic trees of eight genes between
these two viruses (BJ3426 and
SX1616) and some representative isolates obtained from GISAID and
GenBank. The isolates in this study are marked with black squares, and
H7 Re-2 vaccine is marked with black diamonds. The tree was inferred by
MEGA 6.0 using the Maximum Likelihood method based on the Tamura-Nei
model with 1000 bootstrap replicates.
Figure 2. Solid-phase receptor-binding assay of
these two viruses, human isolate
A/California/04/2009 (CA04) and poultry isolate A/mallard/Huadong/S/2005
(HD05). The control viruses of CA04 and HD/05 showed an absolute
preference for human-type (SAα-2,6Gal) and avian-type (SAα-2,3Gal),
respectively. The data shown are representative of three independent
binding experiments.
Figure 3. Thermal and acid stability results of these two
isolates. (A) Mean
haemagglutination (HA) titers of these two viruses (n = 3)
after incubated at 56°C for various
time intervals. (B) Mean virus
titers after incubated at 56°C for various time intervals. (C) Mean
virus titers after incubated at different pH.
Figure 4. Pathogenicity of two H7N9 avian influenza viruses in
mice: mortality based on the survival rate (%) and morbidity measured
by weight change in mice infected with the two tested viruses at the
dose of 106.5EID50. Mice were humanely
killed when they lost ≥25% of their initial body weight.