Cell culture
Raw264.7 (RRID:CVCL_0493) and THP-1 (RRID:CVCL_0006) were obtained from China Center for Type Culture Collection. The cell lines used in this study were authenticated using short tandem repeat (STR) analysis and regularly tested for mycoplasma. Raw264.7 were seeded in 6-well plates (1×106 per well) and cultured with Dulbecco’s Modified Eagle’s medium (HYCLONE, Cat#SH30022.01B), 10% fetal bovine serum (ScienCell, Cat#0500, USA). After 24h, Culture media was then renewed and cells were treated by ox-LDL (Yiyuan Biotechnologies, Cat#2018-05-15,) at a concentration of 50 μg ml-1with or without As2O3(2.5 μM or 5.0 μM) for 24 h. THP-1 were seeded in 6-well plates (1×106 per well) and cultured with 1640 medium (Gibco, Cat#88365), 10% fetal bovine serum and PMA(100nM). After 12 h, culture media was renewed and cells were treated by ox-LDL at a concentration of 50 μg ml-1 with or without As2O3(2.5 μM or 5.0 μM)for 24 h. Cells were then collected for detection.