Experimental Approach
ApoE-/- mice were fed a high-fat diet for 3 months and treated with ATO every alternate day for 30 days. Carotid artery and serum samples were collected to determine atherosclerotic lesion size, histological features, and related protein and lipid profiles. In vitro , RAW264.7 or THP-1 cells were stimulated using oxidised low-density lipoprotein (ox-LDL) or LPS to explore the anti-inflammatory and anti-pyroptosis effects of ATO.