Experimental Approach
ApoE-/- mice were fed a high-fat diet for 3 months and
treated with ATO every alternate day for 30 days. Carotid artery and
serum samples were collected to determine atherosclerotic lesion size,
histological features, and related protein and lipid profiles. In
vitro , RAW264.7 or THP-1 cells were stimulated using oxidised
low-density lipoprotein (ox-LDL) or LPS to explore the anti-inflammatory
and anti-pyroptosis effects of ATO.