Measurements of cytokines
Serum were collected and determined via ELISA according to the
manufacturer’s instructions. Serum levels of IL-6, IL-10, and TNF-α were
measured by IL-6 (R&D systems,
Cat#DY406-05), IL-10 (R&D systems, Cat#DY417-05) and TNF-α (R&D
systems, Cat#DY410-05) detection kits. The levels of IL-1β in the THP-1
cell supernate were measured by
IL-1β ELISA kit (BOSTOR, Cat#EK0392).