Cell culture
Raw264.7 (RRID:CVCL_0493) and THP-1 (RRID:CVCL_0006) were obtained
from China Center for Type Culture Collection. The cell lines used in
this study were authenticated using short tandem repeat (STR) analysis
and regularly tested for mycoplasma. Raw264.7 were seeded in 6-well
plates (1×106 per well) and cultured with Dulbecco’s
Modified Eagle’s medium (HYCLONE, Cat#SH30022.01B), 10% fetal bovine
serum (ScienCell, Cat#0500, USA). After 24h, Culture media was then
renewed and cells were treated by
ox-LDL (Yiyuan Biotechnologies,
Cat#2018-05-15,) at a concentration of 50 μg ml-1with or without As2O3(2.5 μM or 5.0 μM) for 24 h. THP-1
were seeded in 6-well plates (1×106 per well) and
cultured with 1640 medium (Gibco,
Cat#88365), 10% fetal bovine serum
and PMA(100nM). After 12 h,
culture media was renewed and cells were treated by ox-LDL at a
concentration of 50 μg ml-1 with or without
As2O3(2.5 μM or 5.0 μM)for 24 h. Cells
were then collected for detection.