Figure 6. Proposed model describing the mechanism of
atheroprotection by ATO.
The black arrows represent promotion; The red arrows with flat end
represent inhibition.
Figure Supplement.
TableS1: Primers used in this study.
Figure S1. (A-B) Haematoxylin staining of
ApoE-/- mice heart, liver, spleen, lung and kidney
tissues. (C) Body weight was measured every day from day 1. (D-G) After
the last treatment, serum levels of ALT(D), AST(E), UA(F) and CK(G) were
determined. (H) Quantification of
CD68 on IL-1β positive macrophages in the aortic root.(I) Quantification
of CD68 on Tunel positive macrophages in the aortic root.n = 10 mice in
each group. The data are presented as the mean ± SEM.
*p < 0.05, **p < 0.01 vs control
group.
Figure S2. (A)Flow
cytometry was used to detect the apoptosis of spleen and lymph node
cells in ApoE-/- mice, where
annexin-V+PI- represents early
apoptosis. (B)Flow cytometry was used to detect the percentages of T, B,
and monocytes in peripheral blood of ApoE-/- mice, and
were characterized by CD3, Ly6G, and B220, respectively. (C)
Quantification of lymphocytes in PBMC of control and ATO groups. (D)
Quantification of apoptosis cells in lymph node and spleen between the
control and ATO groups. n = 5 mice in each group.