Figure 6. Proposed model describing the mechanism of atheroprotection by ATO.
The black arrows represent promotion; The red arrows with flat end represent inhibition.
Figure Supplement.
TableS1: Primers used in this study.
Figure S1. (A-B) Haematoxylin staining of ApoE-/- mice heart, liver, spleen, lung and kidney tissues. (C) Body weight was measured every day from day 1. (D-G) After the last treatment, serum levels of ALT(D), AST(E), UA(F) and CK(G) were determined. (H) Quantification of CD68 on IL-1β positive macrophages in the aortic root.(I) Quantification of CD68 on Tunel positive macrophages in the aortic root.n = 10 mice in each group. The data are presented as the mean ± SEM. *p  < 0.05, **p  < 0.01 vs control group.
Figure S2. (A)Flow cytometry was used to detect the apoptosis of spleen and lymph node cells in ApoE-/- mice, where annexin-V+PI- represents early apoptosis. (B)Flow cytometry was used to detect the percentages of T, B, and monocytes in peripheral blood of ApoE-/- mice, and were characterized by CD3, Ly6G, and B220, respectively. (C) Quantification of lymphocytes in PBMC of control and ATO groups. (D) Quantification of apoptosis cells in lymph node and spleen between the control and ATO groups. n = 5 mice in each group.