Novel object recognition test
7 days after LPS treatment, the novel object recognition test (NORT) was
applied to examine cognitive function in mice(W. Zhang et al., 2020).
Mice were handled 6 days before the test, for 1 minute a day. Then,
putting mice into the box for 5 minutes to acclimatization. 24 hours
later, mice were allowed to explore two identical rectangular blocks for
5 minutes. After 2 hours, a cylinder was used to replace one of the
rectangular blocks, and mice explored for another 5 minutes. Exploratory
behaviors were performed as sniff, lick and climb. Discrimination ratio
was used to assess learning and memory.