Enzyme-linked immunosorbent assay (ELISA)
Serum samples were collected and centrifuged for 10 minutes at 3000g at
4 °C to obtain supernatants. Expressions of IL-6, IL-1β, and TNF-α were
determined using commercially available ELISA kits (MDL Biotech,
Beijing, China). Concentrations of
inflammation factors were tested
according to the manufacturer’s instructions and presented as pg/ml of
serum protein.