Experimental design
Mice were divided into four groups: Control group, LPS group,
LPS+7,8-DHF group and LPS+7,8-DHF+ANA12 group. In the LPS group, mice
were intraperitoneally injected with LPS at a concentration of 0.25
mg/kg. In the Control group, mice were intraperitoneally injected with
the same amount of saline. In the LPS+7,8-DHF group, 7,8-DHF (5 mg/kg)
were injected 30 minutes before LPS treatment. And in the
LPS+7,8-DHF+ANA12 group, ANA12 (0.5 mg/kg) were injected 30 minutes
before 7,8-DHF and LPS. The novel object recognition test was performed
to detect cognitive function 7 days after treatment.