Figure 4. PLSCR1 binds ILDR1. (A) Western blots showing that EGFP-tagged cytoplasmic fragment of ILDR1 was co-immunoprecipitated with Myc-tagged PLSCR1. (B) Western blots showing that EGFP-taggedPLSCR1 was co-immunoprecipitated with Myc-tagged cytoplasmic fragment of ILDR1. Expression vectors were transfected into HEK293T cells to express epitope-tagged proteins, and cell lysis were subject to immunoprecipitation.5% of total protein was loaded as input. IP indicates antibody used for immunoprecipitation and WB indicates antibody used for detection. (C) ILDR1-EGFP localize in the cytoplasm of COS7. (D) PLSCR1-mCherry localize in the cytoplasm of COS7. (E) However, when PLSCR1-mCherry is present, ILDR1-GFP translocates into the nuclei. Expression vectors were transfected into COS-7 cells to express epitope-tagged proteins. Nuclei were stained with DAPI. Scale bar: 10 μm.