Figure 3. PLSCR1 influences the infection of mice by Swine influenza
virus (SIV). (A) C57BL/6J and Plscr1_/_ mice
were infected intranasally with 103 pfu. SIV. Suviral
was determined daily and calculated as a percentage of the initial total
numbers. (B)Mice were weighed daily and the weights represented as a
percentage of the starting weight (n=7 per group). (C) Lung tissues were
taken at multiple days p.i. as indicated and virus titer determined by
plaque assay(n>6 per group). Data represent the mean
value±SD. Asterisks indicate statistical difference (Unpaired t test,
*P<0.05, **P<0.01, ***P<0.001). (D)
Western blots showed that ILDR1 and NP protein expression in lung of
C57BL/6 and Plscr1_/_ mice on day 42 after
infected with 103 p.f.u. SIV for 3 days . (E)
Statistical analysis of ILDR1 and NP levels inPlscr1_/_ mice. The value for ILDR1 and NP
were standardized to the GAPDH level and normalized to the level of
ILDR1 and NP in lung of wild type mice. Data are shown
as the means SD from three independent experiments(Unpaired t test,
*P<0.05, **P<0.01, ***P<0.001).