5. Conclusion
The measurement of NO levels is a sufficient and rational option to begin the trial of anti-inflammatory and immunomodulatory action of any compound or product using the RAW 264.7 model as an experimental platform. However, future experiments need to follow some rules of standardization. In this context, our study suggests a basic checklist to help researchers in future experiments with NO and cytokines using RAW 264.7 macrophages, which can help prevent the unnecessary use of animals and waste of resources.