The measurement of NO levels is a sufficient and rational option to
begin the trial of anti-inflammatory and immunomodulatory action of any
compound or product using the RAW 264.7 model as an experimental
platform. However, future experiments need to follow some rules of
standardization. In this context, our study suggests a basic checklist
to help researchers in future experiments with NO and cytokines using
RAW 264.7 macrophages, which can help prevent the unnecessary use of
animals and waste of resources.