Figure 1. Preferential suppression of fibroblast activity by eperisone.
LL29 or A549 cells were incubated with the indicated concentrations (µM) of eperisone for 24 h. The percentage of viable cells was determined using a CellTiter-Glo® 2.0 assay (A). LL29 cells were incubated with the indicated concentrations (µM) of eperisone for 18 h. The cytotoxicity was measured every hour using CellTox™ Green Dye and a microplate reader (excitation: 485 nm, emission: 530 nm) (B). LL29 cells were incubated with transforming growth factor (TGF)-β1 (5 ng/ml) for 72 h in the presence of the indicated concentrations of eperisone. Total RNA was extracted and subjected to real-time RT-PCR using a specific primer set for each gene. The values were normalized to Gapdh gene expression and expressed relative to the control sample (C). Values represent the mean ± SEM ** P < 0.01 ; *P < 0.05 ; NS, not significant.