Ethical approval
All procedures were in accordance with the ethical standards of Helsinki’s declaration after approved by ethical committee of Basic Medical Sciences
Institute, JPMC Karachi, Pakistan (NO.F.2-81-IRB/2017-GENL/418/JPMC). Pakistan.
Patient consent; the written and verbal consents were obtained for the research.
Competing interest : The authors declare that they have no conflict of interest.
Funding: none
Acknowledgements: none