Measurement of IgE, IgG, and IgG4 antibody and peptide
synthesis
Cockroach-specific IgE, IgG, and IgG4 antibody levels were measured in
sera using a Thermo Fisher Scientific ImmunoCAP system (Phadia 250
Immunoassay Analyzer; Thermo Fisher Scientific, Portage, MI, USA) as
explained in the online supporting information. Sequences of 11 major
cockroach allergens were collected from UniProt. 15mer peptides
overlapping by 10 amino acids were generated to get the full coverage of
all sequences (Supplementary Table 1 ). Peptides were purchased
from A & A (San Diego, CA, USA) as crude material on a small (1 mg)
scale.