Measurement of IgE, IgG, and IgG4 antibody and peptide synthesis
Cockroach-specific IgE, IgG, and IgG4 antibody levels were measured in sera using a Thermo Fisher Scientific ImmunoCAP system (Phadia 250 Immunoassay Analyzer; Thermo Fisher Scientific, Portage, MI, USA) as explained in the online supporting information. Sequences of 11 major cockroach allergens were collected from UniProt. 15mer peptides overlapping by 10 amino acids were generated to get the full coverage of all sequences (Supplementary Table 1 ). Peptides were purchased from A & A (San Diego, CA, USA) as crude material on a small (1 mg) scale.