Statistical analysis
Comparisons between cytokine responses were performed using the nonparametric two-tailed, paired Wilcoxon test. Correlations between magnitude of response and polarization or between different cytokine production in each participant were performed using the Spearman’s rank correlation coefficient test. No adjustment for multiple comparisons was performed. Pearson correlation test was used to compare the two different measurements of Tregs. Prism 8.0.1 (GraphPad, city, state, USA) was used for these calculations. Values pertaining to significance and correlation coefficient (R) are noted in the respective figure, and P < 0.05 defined as statistically significant.