Statistical analysis
Comparisons between cytokine responses were performed using the
nonparametric two-tailed, paired Wilcoxon test. Correlations between
magnitude of response and polarization or between different cytokine
production in each participant were performed using the Spearman’s rank
correlation coefficient test. No adjustment for multiple comparisons was
performed. Pearson correlation test was used to compare the two
different measurements of Tregs. Prism 8.0.1 (GraphPad, city, state,
USA) was used for these calculations. Values pertaining to significance
and correlation coefficient (R) are noted in the respective figure, and
P < 0.05 defined as statistically significant.