Correlation of T cell responses with sera antibody titers to CR extract, SPT responses, and clinical presentation
We also tested whether T cell responses were associated with IgE, IgG, and IgG4 titers specific for CR as well as with the ratio between IgE/IgG4 responses. The results summarizing these analyses are shown inTable 2 . A significant correlation was observed between IL-4 responses or IL-4 (Th2) polarization and IgE levels (p=0.0003; R=0.42 and p<0.0001; R=0.45, respectively) and to a less extent with total IgG but not IgG4. Conversely, IFNγ (Th1) and IL-10 polarization showed an inverse correlation with the IgE titers (p=0.0044; R=-0.33 and p=0.0013; R=-0.37, respectively). The same results were noted in regards to the correlation with the IgE/IgG4 ratios. In addition, as shown inTable 2 , the total magnitude of responses positively correlated with IgE titers or the IgE/IgG4 ratios (p=0.0082; R=0.31 and p=0.0071; R=0.32, respectively), and no associations were observed for Treg frequencies.
We next examined the correlation of T cell responses with the results of a skin prick test (SPT) specific for extracts of German and American CR, which share homologous allergens (www.allergen.org). As shown inSupplementary Table 3 , total responses or IL-4 responses were positively but weakly correlated (p=0.035; R=0.25 and p=0.042; R=0.35, respectively), while IL-10 polarization was negatively correlated (p=0.015; R=-0.29) with the mean wheal diameter (MWD) of the SPT for the American CR extract. Conversely, none of the T cell variables correlated with MWD of the SPT for the German CR extract (Supplementary Table 3) . Despite differences in the source of the CR extract considered, these results confirm the associations noted at the serological level.
Lastly, we examined correlations of the T cell variables (magnitude, polarization, or dominance of specific allergens) with clinical variables such as those captured by Composite Asthma Severity Index (CASI) score and components 34. As shown inSupplementary Table 4 , none of the T cell immunological variables correlate with the total CASI score. These results were not unexpected given the fact that this cohort was designed to encompass relatively homogeneous CASI scores, ranging from low to medium severity (median CASI of 4).