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\begin{document}

\title{The Key Mechanism of Radix Isatidis in the therapy of coronavirus
disease 2019 based on network pharmacology and molecular docking}



\author[1]{Zi·Yuan Yu}%
\author[2]{Shengchen Hu}%
\author[3]{Bo-Yuan Chen}%
\author[2]{Dong-Mei Liao}%
\author[2]{Zheng-Yi Zhou}%
\author[4]{Xu-Guang Guo}%
\affil[1]{ Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University, 510150}%
\affil[2]{ Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University,510150}%
\affil[3]{Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University,510150}%
\affil[4]{ Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University,}%


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  \date{May 24, 2024}


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\begin{abstract}
Background Using network pharmacology,systematicly reveals the
Mechanisms of Radix Isatidis in the therapy of coronavirus disease 2019
(COVID-2019). Methods The active components and targets of Radix
Isatidis were obtained by searching the Batman, TCMSP, and
SwissTargetPrediction databases, and COVID-19 related targets were
searched through Genecards, Drugbank, and Malacards database. After
that, we used String database to establish protein-protein interaction,
using David database to conduct enrichment analysis of gene ontology and
pathway enrichment analysis based on the Kyoto encyclopedia of genes and
genomes. The molecular docking was performed using ChemOffice, PyMOL,
and Auto Dock software. Result A total of 73 active ingredients and 1584
related gene targets of Radix Isatidis were screened from kinds of
databases while 559 gene targets of COVID-19 were picked out. Among
them, there are 70 of the same targets. According to the GO enrichment
analysis(P[?]0.01), 35 of them belong to biological process, 13 of them
belong to cell component, and 14 of them belong to molecular function.
And for KEGG analysis, 22 pathways were obtained(P[?]0.01), including PI3K
- Akt signaling pathway andHIF - 1 signaling pathway. Conclusions Radix
Isatidis may act on the PI3K - Akt signaling pathway and HIF-1 signaling
pathway Through direct or indirect regulation of the above goals and
ways to play role in the therapy of COVID-19%
\end{abstract}%



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