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Figure legends :
Figure 1. A,B,C: Demographic profile of the cohort; D: Kaplan
Meier curve depicting the overall survival in patients with ETANTR
versus EBL versus others.
Figure 1S (case #12). A: T1 Sagittal MRI plane showing well
demarcated iso to hypo intense lesion in pons; B: T2 axial MR showing
well defined hyper intense lesion on right side of pons mimicking an
extra axial lesion; C: FLAIR images show ill-defined margins suggesting
an intra axial lesion with possible exophytic component; D: Post
contrast axial MR showing non enhancing lesion expanding the pons on
right side; E: Post contrast sagittal MRI showing non enhancing lesion;
F:3D turbo spin T2 image defining the lesion; G: Diffusion weighted MR
suggesting a cellular tumor; F:T1 coronal MR showing the extent of
lesion.
Figure 2: Embryonal tumor with abundant neuropil and true
rosettes. A: Biphasic histological pattern with cellular islands within
abundant neuropil (H&E, scale bar, 100µm); B: Multilayered rosettes
present within sheets of embryonal cells (scale bar, 50µm); C: Cellular
islands with embryonal cells disposed in sheets, several mitotic figures
and apoptotic debris is noted in the background (scale bar, 20µm); D:
Multilayered rosettes with a central lumen surrounded by mitotically
active cells (scale bar, 20µm).
Figure 3. Embryonal tumor with abundant neuropil and
true rosettes. A: High magnification depicting the characteristic
multilayered rosettes with central lumina showing secretions (scale bar,
10µm); B: Nodular clusters of embryonal cells present within abundant
neuropil (scale bar, 50µm);C: Corresponding section showing diffuse
strong immunoreactivity for LIN 28A within the embryonal cells (scale
bar, 50µm); D: Diffuse strong nuclear expression for INI-1 protein
(scale bar 50µm); E,F: Focal positivity for synaptophysin (E) and GFAP
(F) within the embryonal cells (scale bar, 50µm); ; G: Scant positivity
for Vimentin within the embryonal cells (scale bar, 20µm); H: Ki67
revels a high proliferative index (scale bar, 50µm); I: Ki67 highlights
the multilayered rosettes composed of mitotically active cells around
the central lumen (scale bar, 50µm).
Figure 4 . Ependymoblastoma pattern . A: Sheets of
embryonal cells with numerous multilayered and perivascular rosettes
with scant neuropil stroma in the background (scale bar, 50µm); B: High
magnification depicting closely packed multilayered rosettes (scale bar,
20µm); C: Poorly formed multilayered rosettes (scale bar, 20µm); D:
Strong and diffuse immunoreactivity for LIN 28A within the embryonal
cells (scale bar, 50µm); E: Retained widespread nuclear expression for
INI-1 protein (scale bar, 50µm).
Figure 5: Medulloepithelioma pattern. A: Embryonal cells
disposed in papillary arrangements of neoplastic pseudostratified
neuroepithelium (scale bar, 50µm); B: Sheets of embryonal cells in zones
away from tubular and papillary structures (scale bar, 50µm); C, D: High
magnification depicting pseudostratified neuroepithelium around the
vascular cores (scale bar, 20µm); E: Strong and diffuse immunoreactivity
for LIN 28A within the embryonal cells (scale bar, 20µm); F: Focal, weak
immunoreactivity for cytokeratin (scale bar, 20µm); G: GFAP was not
expressed by the tumor cells (scale bar, 20µm); H: Retained widespread
nuclear expression for INI-1 protein (scale bar, 10µm).