Quantitative real time polymerase chain reaction (qRT-PCR)
The qRT-PCR detection of viral nucleic acid test (NAT) was one of the
most quickly established laboratory diagnosis method in COVID-19
pandemic. After the throat swab specimens in each patient were
collected, the presence of SARS-CoV-2 in throat swab specimens was
detected by qRT-PCR amplification of SARS-CoV-2 open reading frame 1ab
(ORF1ab) and nucleocapsid protein (NP) genes fragments using QIAampRNA
kits. C t value ≦37 was considered positive.