3.1.1 Isolation and Determination of GP
GP were extracted by using the
classical method of hot water extraction followed by ethanol
precipitation. Then, Sevage reagent was used to deproteinate the aqueous
extracts. The crude polysaccharide yield devoid of proteins was found to
be 7.5% of the total dry weight. In addition, the polysaccharides were
dissolved in water after alcohol precipitation, and the water solubility
of the polysaccharides could be greatly increased. The extraction
procedure is shown in Fig. 1A.