5. Conclusions
In the present study, we focused on the identification and quantification of modified peptides by proteomic analysis of dry blood spot (DBS) samples using mass spectrometry. A comprehensive analysis of the samples led to the identification of a total of 976 modified peptides. Among these was a subset of 211 peptides that were found in all samples, indicating their consistent presence and potential biological relevance. The use of mass spectrometry in combination with proteomic techniques enabled the precise identification and quantification of these modified peptides in the DBS samples. This approach provides valuable insight into the diverse range of post-translational modifications that occur in the proteome of DBS samples and highlights their potential importance in various biological processes and diseases. This study will address the detailed characterization and analysis of these identified modified peptides and decipher their specific PTMs and potential impact in the context of the population or disease cohort under study. By elucidating the presence and abundance of these modified peptides, we hope to contribute to the understanding of the molecular mechanisms underlying biological processes and disease development.