5. Conclusions
In the present study, we focused on the identification and
quantification of modified peptides by proteomic analysis of dry blood
spot (DBS) samples using mass spectrometry. A comprehensive analysis of
the samples led to the identification of a total of 976 modified
peptides. Among these was a subset of 211 peptides that were found in
all samples, indicating their consistent presence and potential
biological relevance. The use of mass spectrometry in combination with
proteomic techniques enabled the precise identification and
quantification of these modified peptides in the DBS samples. This
approach provides valuable insight into the diverse range of
post-translational modifications that occur in the proteome of DBS
samples and highlights their potential importance in various biological
processes and diseases. This study will address the detailed
characterization and analysis of these identified modified peptides and
decipher their specific PTMs and potential impact in the context of the
population or disease cohort under study. By elucidating the presence
and abundance of these modified peptides, we hope to contribute to the
understanding of the molecular mechanisms underlying biological
processes and disease development.