RESULTS
Tubeimoside III significantly attenuated the expression of inflammatory
cytokines IL-6, IL-1β, and iNOS in lung and liver tissue homogenates and
RAW264.7 cells. This agent inhibited inflammatory cell infiltration in
alveoli and prevented necrosis in liver lesions in LPS-treated mice.
Extracellular flux analyser assays revealed that tubeimoside III
regulated glucose metabolism in RAW264.7 cells. Real-time quantitative
PCR and western blot revealed that tubeimoside III had similar effects
on the downstream effector molecule of itaconic acid. An inhibitor
weakened the inhibitory effect of tubeimoside III on the expression of
inflammatory factors.