3.2 Effect of Tubee on mRNA expression of inflammatory factors in mice organs
The mRNA levels of inflammatory factors IL-1β, IL-6, TNF-α, and iNOS were significantly higher in the liver, spleen, and lung of mice in the model group, indicating that all organs in the model group had different degrees of inflammatory responses (Figure 3A-D). IL-6, and iNOS were significantly lower in the liver, spleen, or lung of mice that were injected intraperitoneally with Tube compared with the model group, as well as IL-1β in liver and lung. However, the mRNA levels of TNF-α were not significantly different across organs.