3.6 The effect of Tube on reprogramming sugar metabolism in RAW 264.7 cells
Tube inhibited LPS-induced glycolysis in RAW264.7 macrophages (Figure 8 ) and reduced basal and maximal respiration (Figure 9 ). This finding suggests that Tube inhibits glycolysis in RAW264.7 macrophages at the level of mitochondrial oxidative phosphorylation. The transcript levels of critical glycolytic rate-limiting enzyme genes were measured using RT-PCR. Tube increased the transcript levels of GLTU1, PFKFB3, and hexokinase 2 (HK2), while inhibiting the transcript levels of LDHA (Figure 10 ). Pyruvate content in RAW264.7 macrophages increased with the application Tube (Figure 11 ).