2.1 Animal
Male C57BL/6 mice at 7–10 weeks, weight 20–25 g, were purchased from
Xi’an Jiaotong University Experimental Animal Center (originally from
the Jackson Laboratory), animal number JAX strain code 000664. The mice
were maintained at 24–26 °C, relative humidity of 45%–60%, and a
diurnal cycle time of 12:12 hours. They were permitted access to food
and water ad libitum. The mice were randomly divided into three groups
of ten each, group 1: phosphate-buffered saline (PBS) treatment (Control
group); group 2: 10 mg/kg of LPS treatment (LPS group); group 3: 10
mg/kg LPS + 1 mg/kg Tube treatment (Tube group). The Institutional
Animal Care and Use Committee approved the study, and the animal welfare
regulations established by the Laboratory Animal Center of Xi’an
Jiaotong University were strictly observed.