3.3 Effect of Tube on the viability and apoptosis in RAW264.7
cells
RAW264.7 macrophages in the logarithmic growth phase were resuspended
and plated on 12-well plates at 15×104 cells per well.
The cells were divided into two groups. The first group was treated with
various concentrations of Tube (0, 1, 4, and 7.5 μM), and the second was
treated with Tube 4 μM for 0, 3, 6, and 12 hours. Tube had no
significant effect on the morphology (Figure 4 ) or apoptosis
(Figure 5 ) in RAW264.7 macrophages below 7.5 μM or at 12 h of
action.