METHOD
A mouse model of LPS-induced acute inflammation was constructed, and the
protective effect of tubeimoside III against LPS-induced injury was
investigated using histochemistry and real-time quantitative PCR.
Western blotting, Seahorse extracellular flux analyser assays, and
pyruvate content assays were used in LPS-induced RAW264.7 cells to
explore how tubeimoside III exerts its anti-inflammatory effects. The
potential mechanism was also validated using inhibitors.