3.1 Effect of Tube on the gross morphology and histology in mouse organs
As shown in Figure 1 , in the LPS-induced acute inflammation model (see reference[7] for the concentration of LPS), the spleen of the model group was significantly congested with a dark color and blunted rim compared, indicating hyperfunction after LPS treatment. In contrast, the Tube group had less congestion and a relatively lighter color than the model group; however, the overall appearance was similar to that of the control group. This finding suggests that Tube prevents LPS-induced splenic congestion.
The Tube group had significantly fewer erythrocytes in the splenic red marrow, fewer macrophages in the germinal centers, less inflammatory infiltration of the alveolar wall in the lungs, reduced alveolar wall thickness, and reduced structural damage, suggesting that Tube significantly attenuated LPS-induced histomorphological changes in the spleen and lungs, as shown in Figure 2 . In addition, the LPS sets by looking at Figures 1 and 2 also show that our model construction was successful.