Quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay
According to the manufacturer, total RNA was isolated from RAW264.7 cell by TRIzol (Cwbio). RNA was translated into cDNA using PrimeScript RT Master Mix(Takara Bio). The qRT-PCR was run on the LightCycler® 480 Software using TB Green® Premix Ex Taq™ II (TaKaRa, Bio) with gene specific primers. Gene expression level was calculated employing the 2−ΔΔCt method. The primer sequences were listed inTable S1 .