Quantitative reverse transcription polymerase chain reaction
(qRT-PCR) assay
According to the manufacturer, total RNA was isolated from RAW264.7 cell
by TRIzol (Cwbio). RNA was translated into cDNA using PrimeScript RT
Master Mix(Takara Bio). The qRT-PCR was run on the LightCycler® 480
Software using TB Green® Premix Ex Taq™ II (TaKaRa, Bio) with gene
specific primers. Gene expression level was calculated employing the
2−ΔΔCt method. The primer sequences were listed inTable S1 .