GO analyses of Differentially expressed genes.
The significant DE genes between the TB model group and the normal group or each ag85a/b DNA vaccine group and TB model group were subjected to GO terms for the BP, CC, and MF by GO analyses, and the top 10 significant BP, CC, and MF items enriched in each group were compared (Supplementary Fig. 2). The results showed that: (1) In the TB model group, the up-regulated BP items were mainly enriched in the secretion and regulation of cells, proteins, insulin and hormones, etc; the down-regulated BP items were mainly enriched in locomotion, developmental process, and cell migration, etc. The up-regulated CC items were mainly enriched in the host cell cytoplasm, host intracellular part, neuronal cell body, and secretory granule, etc; the down-regulated CC items were mainly enriched in extracellular matrix part, extracellular region, plasma membrane, and the vesicle, etc. The up-regulated MF items were mainly enriched in ligand activation, G-protein coupled receptor binding, calcium ion binding, peptidase activity, and hormone activity, etc. the down-regulated MF items were mainly enriched in binding, protein binding, ion binding, and receptor binding, etc. (2) Among the top 10 significantly up-regulated or down-regulated GO items in the TB model group, the 10μg and 50μg DNA IM groups had little effect on them, most up-regulated GO items were significantly down-regulated, and most down-regulated GO items were significantly up-regulated in 100μg, 200μg DNA IM groups, and 4 DNA EP groups.