Elevated glucose concentration attenuates DC phagocytosis
By coculturing the inactivated BMDC with Fluoresbrite carboxylate
microspheres and staining the cell nucleus, we next the impact of
glucose on DC phagocytosis capability using fluorescent microscopy. A
reduction in inactivated BMDC phagocytosed-Fluoresbrite microspheres was
observed with the increase in glucose concentration from 5.5 mM to 25 mM
(Figure 4. A – C). Quantification of relative fluorescent microsphere
to cell nucleus ratio significant decreased from 1.2 to 0.5 according to
glucose concentrations (Figure 4. D).