Figure 3. Relative gene expression of glycolysis genes HK2, LDHA, and GLUT1 in inactivated and AGE & LPS-activated BMDC (A – C) and human monocyte differentiated-mDC (D – F) in 5.5-, 11-, and 25-mM glucose. Values were normalized to mRNA level of inactivated mDC in 11 mM glucose. (ns = not significant, *P\(<\)0.05, **P\(<\)0.01, ***P\(<\)0.001). Overall rate curves of metabolic flux of inactivated monocyte differentiated-DC (G) and AGE & LPS-activated monocyte differentiated-DC (H) in 5.5-, 11-, and 25-mM glucose (plated in 75000 cells/well). Glycolysis rate (I) and glycolytic capacity (J) of DC. Data demonstrated in units of extracellular acidification rate (ECAR). (**P\(<\)0.01, ***P\(<\)0.001). Lactate production (K) and OXPHOS activities (L) of inactivated and AGE & LPS-activated BMDC in 5.5-, 11-, and 25-mM glucose. (ns = not significant, *P\(<\)0.05).
Figure 4.
A B C