Figure 1. Immunofluorescence image of inactivated mDC in 5.5- (A), 11- (B), and 25 mM glucose (C); and immunofluorescence image of AGE & LPS-activated mDC in 5.5 mM glucose-(B), 11- (D) and 25 mM glucose (F). The cell nucleus was stained with Hoechst dye (blue), the F-actin was stained with TRITC-conjugated phalloidin (red), and the CD83 was stained with FITC-conjugated antibody (green). Images were captured at 40\(\times\) magnification. Magnified areas from the respective images are displayed on the top right corner to demonstrate cell morphologies and dendrites formation.
Figure 2.
A B C