High glucose modulates DC morphology
Impact of hyperglycemia (11- or 25-mM glucose) was examined on inactivated or P. gingivalis LPS and AGE-product activated mDC differentiated from human monocytes using immunofluorescence staining. At the glucose concentration of 5.5 mM, inactivated mDCs had a spherical or elliptical morphology with few dendrites (Figure 1A). Without stimulation, or by increasing the glucose concentrations alone increased dendrites on the surface of cells, indicating cell spread and maturation (Figure 1. C, E). However, in presence of LPS and AGE, dendrites formation on activated mDC was stimulated in all three glucose concentrations, yet the cells in 11- and 25-mM glucose had more extended dendrites in comparison to the 5.5 mM glucose culture (Figure 1. B, D, F). Both inactivated and activated mDCs in all three glucose concentrations expressed CD83 (Figure 1. A-F).