DC phenotypes are modulated under hyperglycemia
To assess the impact of glucose on BMDC activation, we next performed
RT-qPCR for surface markers CD80, CD86, CD83, and MHC-II complex.The relative mRNA expressions are reported as fold change in comparison
to the inactivated cells cultured in 11 mM glucose (Figure 2. A – D).
The co-stimulatory markers CD80, CD83, and MHC-II were significantly
upregulated in the presence of AGE and LPS in 11 mM glucose cell
cultures. These three genes’ expression were also increased upon
activation in 25 mM glucose but with this difference was not
statistically significant except for MHC-II (Figure 2. A, C, D).
Elevated CD86 was observed in both 11 mM and 25 mM glucose cultures with
not statistical difference (Figure 2. B). Human monocyte
differentiated-mDC demonstrated comparable findings, which LPS and AGE
significantly upregulated expression of CD80, CD86, and HLA-DR (Figure 2
E – H). Expression level of CD83 was not statistically different
(Figure G).