High glucose modulates DC morphology
Impact of hyperglycemia (11- or 25-mM glucose) was examined on
inactivated or P. gingivalis LPS and AGE-product activated mDC
differentiated from human monocytes using immunofluorescence staining.
At the glucose concentration of 5.5 mM, inactivated mDCs had a spherical
or elliptical morphology with few dendrites (Figure 1A). Without
stimulation, or by increasing the glucose concentrations alone increased
dendrites on the surface of cells, indicating cell spread and maturation
(Figure 1. C, E). However, in presence of LPS and AGE, dendrites
formation on activated mDC was stimulated in all three glucose
concentrations, yet the cells in 11- and 25-mM glucose had more extended
dendrites in comparison to the 5.5 mM glucose culture (Figure 1. B, D,
F). Both inactivated and activated mDCs in all three glucose
concentrations expressed CD83 (Figure 1. A-F).