T cell function is impaired when primed with DC cultured in
hyperglycemic conditions
We co-cultured mouse BMDC with mouse splenic T cells to investigate the
impact of hyperglycemia on DC priming and T cell functions. T cells
incubated with BMDC in the absence of AGE and LPS gradually reduced the
levels of pro-inflammatory cytokines, IFN-\(\gamma\) and IL-17, which
was inversely relative to the increasing glucose concentration (Figure
6. A, C). AGE and LPS resulted in activation of DC that further
activated T cells. In comparison to the unstimulated cells, levels of
both cytokines significantly increased in all glucose concentrations
upon AGE and LPS-stimulation. Cytokines released from these activated
cells showed an inverse correlation with the glucose concentrations. The
level of IL-6 from T cells also decreased with increasing glucose
concentration in the unstimulated culture. However, upon stimulation,
IL6 significantly increased to approximately 1020 pg/mL in 5.5 mM, 11
mM, and 25 mM glucose concentrations. (Figure 6. B).