Elevated glucose concentration attenuates DC phagocytosis
By coculturing the inactivated BMDC with Fluoresbrite carboxylate microspheres and staining the cell nucleus, we next the impact of glucose on DC phagocytosis capability using fluorescent microscopy. A reduction in inactivated BMDC phagocytosed-Fluoresbrite microspheres was observed with the increase in glucose concentration from 5.5 mM to 25 mM (Figure 4. A – C). Quantification of relative fluorescent microsphere to cell nucleus ratio significant decreased from 1.2 to 0.5 according to glucose concentrations (Figure 4. D).