2.1 Preparation of HPMEC
HPMEC was purchased from Guangzhou Dewei Biological Technology Co. Ltd,
China. HPMEC was cultured in the endothelial cell medium with 10% fetal
bovine serum (ScienCell, America) at 37 °C in a humidified incubator
with 5% CO2. All experiments were carried out during
the logarithmic phase of cell growth. The account of cells was adjusted
to 1x106 /ml by serum-free medium before culture,
inoculating into six-well plates, and incubated at 5%
CO2 and 37 °C.