Figure 2- Flow Cytometry analysis of CD4+T cells subsets-the bar graphs represent the comparison of percentages of immune cells and their subpopulation at different time point A] CD4+ Naïve T cells B] CD4+ Effectors T cells C]CD4+ Central Memory T cells and D] CD4+ effector Memory T cells profile. Data are represented as median with interquartile range. *P<0.05, **P<0.001 and *** P <0.0001 significant.
We observed that CD8+ T cells showed more sustained changes in their naive and central memory from one month to 6-8 months after enrolment (Figure 3A & 3C). The frequencies of CD8+ effectors and CD8+ T Effector Memory, on the other hand, increased from one month to 6-8 months (Figure 3B & 3D). Overall, we found alterations in the memory T cell pool, most notably in the CD4+ and CD8+ effector memory subsets, where the changes persisted until the end of the study.