2.10 Statistical analysis
Differences among treatments were analyzed with one-way ANOVA followed by Duncan’s multiple range test to compare treatments (or a Student’st -test when only two treatments were compared). We established the filtering criteria correlation between differentially expressed transcripts and differentially directed m6A methylation in Ni-Nip vs. Nip group as satisfying both differentially expressed transcripts p -value < 0.05 (based on either RT-qPCR or transcriptome), and a significant m6A methylation direction p -value < 0.05 and |meth diff| > 10.