Results
A cartoon illustrating the anatomy of the CVC in T. thermophila ,
together with a small set of proteins whose distributions were
determined by in vivo localization studies of endogenously tagged
genes, is shown in Fig. 1A. As previously reported (Sparvoli et al.,
2020, Cheng et al., 2023), the CORVET subunit Vps8D, here endogenously
tagged with mCherry, localizes to both the bladder and spongiome (Fig.
1A, right). Similarly, endogenously tagged CORVET subunit Vps11p
localizes to the CVC, but also to other cellular loci as expected since
it is a subunit of multiple CORVET complexes in this organism (Sparvoli
et al., 2020) (Fig. S1).