MATERIALS AND METHODS
Specimen examined: Eggs (N=80) Muğla Province, Dalaman district,
Kapıgargın Village, Kocagöl, lake shore, 36º41’N 28º50’E, altitude: 4 m,
15.07.2001.
A male and a female of the Lindenia tetraphylla species were
captured while mating on the lakeshore in Kocagöl using an aerial net.
Once the mating process was completed, the female was held by her wings.
In a jar filled with lake water, the genital area of the female’s
abdomen was stimulated to induce egg-laying behavior, and she was then
placed in the water to lay eggs. After approximately one hour, the water
was slowly replaced with 70% ethanol to kill and preserve the eggs. One
week later, the ethanol was replaced with fresh ethanol, and the samples
were stored in a dark environment.
For the scanning electron microscopy examinations, the eggs were
dehydrated in ascending series of ethyl alcohol and air dried. Dried
specimens were mounted by double-sided adhesive tape on SEM stubs and
coated with gold in sputter coater (Polaron SC 502). The coated samples
were examined with JEOL JSM 6060 LV SEM at accelerating voltage 15 kV
and digital photographs were taken at Gazi University, Faculty of
Science, Prof. Dr. Zekiye Suludere Electron Microscopy Center.