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\begin{document}

\title{Impact of Nanoplastic Contamination on Rhizosphere Microbiome and Plant
Phenotype}



\author[1]{Henrique Petry Feiler}%
\author[1]{Sara Norato}%
\author[1]{Leilei Zhang}%
\author[1]{Luigi Lucini}%
\author[1]{Yang Yang}%
\author[1]{Sungchan Oh}%
\author[1]{Lori Hogland}%
\affil[1]{Affiliation not available}%


\vspace{-1em}



  
  \date{June 06, 2024}


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\begin{abstract}
Nanoplastic (NP) is an organic contaminant that is widespread in soil,
water, and food. However, the effects of NP are not well understood,
especially in the context of the rhizosphere-roots-microbiome interface
and how they can impact both plants and the soil microbiome. Our
hypothesis is that the presence of NP in the soil will lead to distinct
changes in the root microbiome and result in a unique phenotype in the
plant. To investigate this, we conducted an experiment in which two
crops, tomato (\emph{Solanum lycopersicum} cv. Micro-tom) and lettuce
(\emph{Lactuca sativa} L. cv Canasta), were planted in three different
soil conditions: a control group with no NP (zero-NP) and two
experimental groups with NP concentrations of 25 and 250
mg.kg\textsuperscript{-1}. The experiment took place over a 41-day
period at Purdue's Ag Alumni Seed Phenotyping Facility. During this
time, manual plant measurements and red-green-blue (RGB) and
hyperspectral imaging were performed on 17 different dates. After the
41-day growth period, the plants were harvested and weighed, soil from
pots and subject to various enzymatic assays to quantifying difference
in elemental cycling potential, and DNA was extracted from both the bulk
soil and the rhizosphere+roots. The 16S and ITS rRNA genes were then
amplified and sequenced using the MiSeq Illumina technology and subject
to various bioinformatic programs to quantify differences in composition
and functional potential. This study aims to provide insights into how
NP affects the rhizosphere, plants, and the associated microbiome, and
the results may shed light on the environmental implications of NP
contamination.%
\end{abstract}%



\sloppy


\emph{\textbf{Henrique Petry Feiler\textsuperscript{1}}, Sara
Norato\textsuperscript{2,3}, Leilei Zhang\textsuperscript{4}, Luigi
Lucini\textsuperscript{4}, Yang Yang\textsuperscript{5}, Sungchan
Oh\textsuperscript{5}, Lori Hogland\textsuperscript{\textbf{2}}}

\emph{\textsuperscript{1}Departament of Agronomy, Purdue, Lafayette,
EUA}

\emph{\textsuperscript{2}Departament of Landscape and Horticulture,
Purdue, Lafayette, EUA}

\emph{\textsuperscript{3}National University of Colombia, Bogota,
Colombia}

\emph{\textsuperscript{4}Catholic University of the Sacred Heart,
Piacenza, Italy}

\emph{\textsuperscript{5}Plant Science Institute, Purdue University}

\textbf{ORCiD:} 0000-0001-7848-7136

\textbf{Keywords:} Microplastic, bacteria, fungi

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