Statistical analysis
Excel 2019, SPSS 26.0, GraphPad Prism 8.0 was used to describe and analyze the data statistically. The continuous variables were run by one way Kolmogorov-Smirnov test to determine distribution patterns, then means and standard deviations were used to represent which are normal while medians and interquartile ranges were used to represent which are skew. Qualitative data are displayed as numbers and percentages. Student t test was used to compare normal continuous variables, Mann-WhitneyU test were used to compare skew continuous variables, while χ2 test or Fisher’s exact test was used to compare categorical variables. Spearman correlation analysis were used to calculate correlations between different groups of variables. The p-value for statistical significance was set at 0.05.