2.6.7.Analysis of the cell subpopulations
The function of FindAllMarkers in R package Seurat was used for signature genes selection of each cell subpopulation. The differential genes among subpopulations were filtered by: adjusted p-value <0.05, log2(foldchange) ≥1, differences between pct.1 and pct.2 ≥0.3. The annotations of cell populations were identified through a combination of published marker genes and functional characterization of highly variable genes. Then, R packages clusterProfiler and irGSEA were applied to estimate the enrichment scores of the cell subpopulations . The visualization was completed by R package scRNAtoolVis.