2.6.7.Analysis of the cell subpopulations
The function of FindAllMarkers in R package Seurat was used for
signature genes selection of each cell subpopulation. The differential
genes among subpopulations were filtered by: adjusted p-value
<0.05, log2(foldchange) ≥1, differences between pct.1 and
pct.2 ≥0.3. The annotations of cell populations were identified through
a combination of published marker genes and functional characterization
of highly variable genes. Then, R packages clusterProfiler and irGSEA
were applied to estimate the enrichment scores of the cell
subpopulations . The visualization was completed by R package
scRNAtoolVis.