3.7 Annotations of differentially expressed genes (DEGs)
Compared with control, differentially expressed genes (DEGs) were
identified in the 200 mg/L Mt and Pal treatments.
Since photosynthesis is an
important physiological process in algae, we focused on those
significantly different genes involved in photosynthesis (Figure. 8).
IF2CP and CPLS1 genes were related to chloroplast synthesis based on the
KEGG [35]. After the addition of Pal, these genes were
down-regulated, which inhibited the chloroplast precursor material
conversion to chloroplast. CAB4, CAB7 and CHLG genes regulated
chlorophyll synthesis [36,37]. These genes were significantly
upregulated compared with addition of 200 mg/L Mt. In addition, psbH and
OHP1 genes related to PSII synthesis were significantly downregulated at
Pal200 [38,39]. In addition, odhA and SAMC1 genes
related to cellular respiration were upregulated at
Pal200 [40,41]. However, the change was not evident
in the gene expressions when Mt was added.