3.1 | Three dimensional OsAGPaseL1 and OsS1 modelsĀ
Domain analysis of OsL1 and OsS1 revealed an N-terminal loop-region
followed by two structurally folded functional domains; catalytic domain
and β-helix domain (Figure 1A). Prior to 3D modeling, the N-terminal
loop regions, OsL1 (1-73) and OsS1 (1-55), were removed and structurally
folded regions were considered for model building. As displayed (Figure
1B), both 3D models (OsL1 and OsS1) showed similar structural features
with those of the potato AGPase SS (StSS) crystal structure
(1YP3)29 with a minimum structural deviation (RSMD:
0.409 for OsL1 vs. StS1, 0.327 for OsS1 vs. StSS). The predicted models
were energy optimized and validated with their stereo-chemical
parameters using several model validation servers (Table S1; Figure S1)
and considered for further structural study.