Contrast enhanced-ultrasound
This analysis was performed at the beginning of the protocol (W0) and after one week (W1). Mice were anesthetized with 2% isoflurane. Pre-warmed echo transmission gel was applied to the tumor mice. Acquisition time did not exceed 80 seconds. For each tumor, at least 3 measures corresponding to length (L), width (w) and height (h), were made by ultrasonography imaging with an Arietta v70 equipment (Hitachi Medical Systems, Saint-Priest, France). The volume (V) in mm3 was calculated considering tumors as semi-ellipsoids, with the formula V = (4/3 π x L x w x d) /2 (w, L and d, are the tumor width, length and depth, respectively).