Measurement of tumor volume using a calliper or CEUS
Monitoring of tumor volume with caliper showed no significant difference between anti-PD1 treated mice and control mice (Figure 2.A ). Moreover, the measurement of tumor volume through CEUS did not show any difference between control and anti-PD1 treated mice at day 0 and after 7 days (Figure 2.B) . In control mice, analysis of tumor volume did not show any significant difference between caliper or CEUS techniques. Likewise, no significant difference was observed in anti-PD1 treated mice. However, although these data are not significant, the distribution of the data appeared to be different. Indeed, the group of mice treated with anti-PD1 and whose tumour volume was measured by caliper showed a wider dispersion of points, indicating greater variability and lower reproducibility for this condition. When tumour volume was measured by ultrasound, the distribution of points was tighter and more consistent, suggesting better reproducibility of the data (Figure 2.C ).