Laser speckle contrast analysis
Measurements were performed twice at W0 and W1 in an air-conditioned and temperature-controlled laboratory. Scanning LDPI measurements were performed by using a PeriScan PIM 3 System 14 and proprietary acquisition LDPIwin software (both by Perimed-Instruments, Järfälla, Sweden). The wavelength with most limited penetration depths into the skin and which is precaunised for healthy skin was choosen, which is the 670 nm laser diode [9]. The distance between laser head and skin chosen by the operator was 12 cm. Acquisition time did not exceed 80 seconds. Mice were anesthetized with 2% isoflurane. Tumoral perfusion was evaluated in different ROI (region of interest) – one on an aluminum patch to avoid artefacts (ROI 1), one in normal skin (ROI 2) and one in tumoral site (ROI 3).
The perfusion was determined with the following equation: (ROI1-ROI3)-(ROI2-ROI3)