Measurement of tumor volume using a calliper or CEUS
Monitoring of tumor volume with caliper showed no significant difference
between anti-PD1 treated mice and control mice (Figure 2.A ).
Moreover, the measurement of tumor volume through CEUS did not show any
difference between control and anti-PD1 treated mice at day 0 and after
7 days (Figure 2.B) . In control mice, analysis of tumor volume
did not show any significant difference between caliper or CEUS
techniques. Likewise, no significant difference was observed in anti-PD1
treated mice. However, although these data are not significant, the
distribution of the data appeared to be different. Indeed, the group of
mice treated with anti-PD1 and whose tumour volume was measured by
caliper showed a wider dispersion of points, indicating greater
variability and lower reproducibility for this condition. When tumour
volume was measured by ultrasound, the distribution of points was
tighter and more consistent, suggesting better reproducibility of the
data (Figure 2.C ).