3.2 Association of gut bacteria and MN
Eight bacteria taxa were observed to be associated with MN according to IVW analysis (Figure 2 and Table 1), seven of which were related to a higher risk of MN, including Class. Melainabacteria (OR:1.51, 95% CI: 1.004-2.277, P = 0.048), Genus. Butyricicoccus (OR: 2.16, 95% CI: 1.005-4.621, P = 0.048), Genus. Catenibacterium (OR: 1.49, 95% CI: 1.043-2.134, P = 0.028), Genus.Ruminiclostridium5 (OR: 1.74, 95% CI: 1.053-2.862, P = 0.030), Genus. Ruminococcaceae UCG-003 (OR:1.73, 95% CI:1.110-2.692, P = 0.015), Order. Bacillales (OR: 1.52, 95% CI:1.135-2.025, P = 0.0048) and Order. Gastranaerophilales (OR:1.45, 95% CI: 1.010-2.085, P = 0.044). Conversely, Genus. Oscillibacter (OR: 0.57, 95% CI: 0.328-0.979,P = 0.042) was associated with a lower risk of MN. The statistical power of the causal inference according to the IVW method on Genus. Butyricicoccus reached to be 0.95, with a type-I error of 0.05. Among these eight bacteria taxa, the Bonferroni-corrected test indicated only the results of the weighted median of Order. Bacillales was significant (Table 1, OR: 1.78, 95% CI: 1.231-2.572; P = 0.002). The reverse causality between gut microbiota and MN suggested statistically significant in Genus.Ruminiclostridium5 and Genus. Ruminococcaceae UCG-003 (Supplementary Table 4). Figure 3 illustrated the correlation between gut microbiota and MN.