Quantification of HBsAg
The supernatant of the cultured cells was harvested at 48 h post-treatment. Levels of hepatitis B surface antigen (HBsAg) in the supernatant were quantified using an HBsAg quantitative determination kit (Shenzhen New Industries Biomedical Engineering Co., Ltd., Shenzhen, China) on a MAGLUMI X3 series automatic chemiluminescence immunoassay analyzer according to the manufacturer’s instructions.