Transcriptional characteristics of integrated HBV DNA in PLC/PRF/5. (A) Quantification of chimeric transcripts derived from different integrated HBV DNA. The counts of chimeric HBV RNA reads originating from different integration sites was used to represent the transcription level of HBV DNA at different integration sites. (B) IGV snapshot of HBV DNA transcription integrated on chr11. Pink is the HBV sequence, light blue is the host sequence, and gray is the junction sequence. Integration of HBV transcription is usually accompanied by the addition of a poly(A) tail in humans, taking HBV integration in chromosome 11 as an example. (C) IGV snapshot of transcription patterns of HBV DNA on chr13.  (D) IGV snapshot of HBV DNA transcription integrated on chr12. The pink color in ONT reads is the HBV sequence (3196~3221/1~458nt), the light blue is the MVK gene exon sequence, the green represents the polyA, and the dark blue is the RNA splicing information. (E) IGV snapshot of HBV DNA transcription integrated on chr3. (F) Schematic representation of two transcription patterns of integrated HBV DNA on chr17.