Genome sequencing
Genomic DNA was extracted by SteadyPure plant genomic DNA extraction kit
(https://agbio.com.cn/) and sequenced on the Illumina HiSeq X Ten
platform and PacBio platform. After quality control of the generated
reads, clean data was obtained. We obtained a total of 107 Gb short
reads and 60.5 Gb long reads on PacBio, respectively.
Fresh rosemary plant leaf tissue was used for creating the Hi-C library.
The tissue sample was cross-linked with formaldehyde for 30 minutes at
room temperature. After purification, the DNA was digested with
restriction enzymes. Following digestion, the fragments were
biotin-labeled, blunt-ended ligated, and DNA was extracted. The purified
DNA was digested into 300– 700 bp fragments, which were used to
construct a DNA library and sequenced on the Illumina HiSeq platform,
yielding a total of 111 Gb reads.