Genome sequencing
Genomic DNA was extracted by SteadyPure plant genomic DNA extraction kit (https://agbio.com.cn/) and sequenced on the Illumina HiSeq X Ten platform and PacBio platform. After quality control of the generated reads, clean data was obtained. We obtained a total of 107 Gb short reads and 60.5 Gb long reads on PacBio, respectively.
Fresh rosemary plant leaf tissue was used for creating the Hi-C library. The tissue sample was cross-linked with formaldehyde for 30 minutes at room temperature. After purification, the DNA was digested with restriction enzymes. Following digestion, the fragments were biotin-labeled, blunt-ended ligated, and DNA was extracted. The purified DNA was digested into 300 700 bp fragments, which were used to construct a DNA library and sequenced on the Illumina HiSeq platform, yielding a total of 111 Gb reads.