Figure 5 Biosynthetic pathway of 1,8-cineole and gene structure analysis
a Downstream pathway of 1,8-cineole biosynthesis and limonene synthase. Intermediates are shown in structural formula with black and bold, and reaction process involved in each step are shown in grey color. Dotted arrows indicate predicted or unknown reaction. The solid boxes mean 1,8-cineole and limonene. b The cluster of 1,8-cineole synthase and limonene synthase in pseudochromosome 3 ofS. rosmarinus . Genes are labeled with italic, arrows present the position and strand of genes. Red arrows represent SrCinSs in cluster, yellow arrows represent SrLSs in cluster, black arrow means the other TPS of the cluster, and blue arrows present the transcription factors of the cluster. c The expression of SrCinSs andSrLSs in three tissues of S. rosmarinus plants. Genes are shown in italic. Red, high expression; blue, low expression. dDocking sites demonstrating active-site amino acid substitutions ofSrCinS-4 (left) and SrCin-5 (right), which highly express in the leaves of S. rosmarinus . Ligand alpha-terpinyl is indicated with a white stick, and residues in active pocket are shown in sticks, helixes are shown in blue, loops are shown in pink. Residues and position are labeled in black and bold. The dotted lines with numbers above in gray and bold indicate the distance of residues and alpha-terpinyl.