Figure 5 Biosynthetic pathway of 1,8-cineole and gene structure
analysis
a Downstream pathway of 1,8-cineole biosynthesis and limonene
synthase. Intermediates are shown in structural formula with black and
bold, and reaction process involved in each step are shown in grey
color. Dotted arrows indicate predicted or unknown reaction. The solid
boxes mean 1,8-cineole and limonene. b The cluster of
1,8-cineole synthase and limonene synthase in pseudochromosome 3 ofS. rosmarinus . Genes are labeled with italic, arrows present the
position and strand of genes. Red arrows represent SrCinSs in
cluster, yellow arrows represent SrLSs in cluster, black arrow means the
other TPS of the cluster, and blue arrows present the transcription
factors of the cluster. c The expression of SrCinSs andSrLSs in three tissues of S. rosmarinus plants. Genes are
shown in italic. Red, high expression; blue, low expression. dDocking sites demonstrating active-site amino acid substitutions ofSrCinS-4 (left) and SrCin-5 (right), which highly express
in the leaves of S. rosmarinus . Ligand alpha-terpinyl is
indicated with a white stick, and residues in active pocket are shown in
sticks, helixes are shown in blue, loops are shown in pink. Residues and
position are labeled in black and bold. The dotted lines with numbers
above in gray and bold indicate the distance of residues and
alpha-terpinyl.