Figure 7. Further analysis of the changes of emission intensity at 512 nm during the FRET experiments, revealing the details of the adsorption and desorption of KE-RhoB. The KE-RhoB concentration was kept constant (0.05 mM) with the increase of the CMD-FAM concentration from 0.05 to 0.5 mM. (A) The fluorescence reduction values at 512 nm correspond to the maximum amount of KE-RhoB adsorbed by CMD-FAM at the initial stage. (B) The desorption ratio is the degree of recovery of emission at 512 nm, corresponding to the regeneration of CMD-FAM.
An artificial chaperone can serve opposite roles, a catalyst at low dosage while an inhibitor at high dosage, in regulating the supramolecular polymerization of peptides. This system undergoes a cascading phase separation process, and the interplay and evolution of electrostatic and hydrophobic interactions between chaperones and peptides are the keys to achieving these processes.
Keywords: artificial chaperone, catassembly, supramolecular polymerization, peptide assembly
Wang Li,a Yang Zhou,a Sheng He,a Congsen Wang,a Peichen Shi,a Suixu Li,a Xinchang Wang,b Liulin Yang,*a Xiaoyu Cao,*a and Zhongqun Tiana
An Artificial Chaperone Serves a Dual Role in Regulating the Assembly of Peptides through Phase Separation