Figure 7. Further analysis of the changes of emission intensity
at 512 nm during the FRET experiments, revealing the details of the
adsorption and desorption of KE-RhoB. The KE-RhoB concentration was kept
constant (0.05 mM) with the increase of the CMD-FAM concentration from
0.05 to 0.5 mM. (A) The fluorescence reduction values at 512 nm
correspond to the maximum amount of KE-RhoB adsorbed by CMD-FAM at the
initial stage. (B) The desorption ratio is the degree of recovery of
emission at 512 nm, corresponding to the regeneration of CMD-FAM.
An artificial chaperone can serve opposite roles, a catalyst at low
dosage while an inhibitor at high dosage, in regulating the
supramolecular polymerization of peptides. This system undergoes a
cascading phase separation process, and the interplay and evolution of
electrostatic and hydrophobic interactions between chaperones and
peptides are the keys to achieving these processes.
Keywords: artificial chaperone, catassembly, supramolecular
polymerization, peptide assembly
Wang Li,a Yang Zhou,a Sheng
He,a Congsen Wang,a Peichen
Shi,a Suixu Li,a Xinchang
Wang,b Liulin Yang,*a Xiaoyu
Cao,*a and Zhongqun Tiana
An Artificial Chaperone Serves a Dual Role in Regulating the Assembly of
Peptides through Phase Separation