3.4.1 BugBase Forecast
BugBase analysis is capable of predicting the phenotypes of prokaryotic
bacteria in environmental samples. In Figure 6-a, the predominant
phenotypes observed in all samples at the end of the experiment were
identified as Gram Negative, Stress Tolerant, Oxygen demand (Aerobic,
Anaerobic, Facultatively Anaerobic), Gram-Positive, Potentially
Pathogenic, Contains Mobile Elements, and Forms Biofilms. The study
showed that the abundance of different phenotypes of microorganisms
changed at different treatment periods. Following the initial MHWs, an
increase in the relative abundance of Potentially Pathogenic and
Gram-Positive bacteria was observed in the HA group. After the 5-day
recovery, the HB group displayed an increase in the relative abundance
of Stress Tolerant and Gram-Positive bacteria. Subsequently, after the
second round of MHWs, the HC group showed an increase in the relative
abundance of Mobile Elements, Forms Biofilms, Stress Tolerant, and
Gram-Positive bacteria. Similar patterns were also observed in the HD
group, while there was an elevation in the relative abundance of
Anaerobic bacteria at the same time.