Semen Sample Collection
Semen sample were collected by masturbation with an ejaculatory
abstinence of 3 to 5 days for all patients. Semen analysis was performed
according to the WHO 2010 guidelines, evaluating sperm volume,
concentration, motility, viability, morphology. Susequently, semen
samples were treated using conventional freezing and vitrification
methods. For each patient, a semen sample was used to perform both
conventional freezing and vitrification. Additionally, a partion of the
sample was treated to separate seminal plasma from pellet for the
molecular and cellular analysys. In more detail, we recruited 20
patients from January to April 2021. The average age of 20 patients was
39 years (Table 1 ) and none of the patients were hospitalized
due to the SARS-CoV-2 infection. The median interval from viral
infection to provide semen samples was 3 months.