Semen Sample Collection
Semen sample were collected by masturbation with an ejaculatory abstinence of 3 to 5 days for all patients. Semen analysis was performed according to the WHO 2010 guidelines, evaluating sperm volume, concentration, motility, viability, morphology. Susequently, semen samples were treated using conventional freezing and vitrification methods. For each patient, a semen sample was used to perform both conventional freezing and vitrification. Additionally, a partion of the sample was treated to separate seminal plasma from pellet for the molecular and cellular analysys. In more detail, we recruited 20 patients from January to April 2021. The average age of 20 patients was 39 years (Table 1 ) and none of the patients were hospitalized due to the SARS-CoV-2 infection. The median interval from viral infection to provide semen samples was 3 months.