Cytometric Bead Array (CBA)
Using the BD Cytometric Bead Array (CBA) Human Kit Flex Set (BD
Bioscience-Pharmingen, San Diego, CA). to measure IL-2 (catalog
#558270), IL-6 (catalog #558276), IFN-γ (catalog #558269), IL-10
(catalog #558274), TNF (catalog #560112), IL-1β (catalog #558279),
IFN-α (catalog #560379), GM-CSF (catalog #558335) and IL-8 (catalog
#558277). The kit contains three bead populations with distinct
fluorescence intensities. The beads were coated with capture antibodies
specific to IL-2, IL-6, IFN-γ, IL-10, TNF, IL-1β, IFN-α, GM-CSF and IL-8
and mixed with phycoerythrin (PE) conjugated detection antibodies. These
capture beads were incubated with recombinant standards or test samples
to form sandwich complexes. The CBA was analyzed at FACSCcanto flow
cytometer (BD Bioscience-Pharmingen). Results were anayzed using graphic
and tabular formate using CBA analysis software (BD
Bioscience-Pharmingen).