Figure 1. AB4 ameliorates DSS-induced colitis symptoms.(A ) Chemical structure of AB4, AA3, and 23-HA. (B-C )
THP-1 cells (pretreated with 500 nM PMA for 3 h) were pretreated with
AB4, AA3, and 23-HA (50μM) for 4h, and then cultured with LPS
(1μg/ml) for 6h. (B ) The
mRNA expression of IL-1β was analyzed by qPCR. (C ) Released
IL-1β in the supernatant was analyzed by ELISA. Data are presented as
mean ± SD. **P < 0.01 vs. Control group;#P < 0.05, ##P
< 0.01 , and ns, not significantly different vs. LPS
group. $$P < 0.01 LPS+AB4vs. LPS+AA3. C57BL/6 mice were intraperitoneally injected with
AB4 (5, 10, and 15mg/kg) for 7 days, and then given 3.0% DSS in
drinking water for 7 days, during which AB4 (5, 10, and 15mg/kg) was
continuously injected intraperitoneally (n = 8 per group). At the end of
the experiment, the mice were sacrificed, and the colons and the spleens
were collected. (D ) The body weight change, (E ) the
disease activity index (DAI), (F ) the colon length,
(G ) the spleen index, and (H ) the survival rate were
measured. Data are presented as mean ± SD. **P < 0.01and ns, not significantly different vs. Normal group;#P < 0.05, ##P
< 0.01 vs. DSS group.