Figure 7. AB4 inhibits the activation of NLRP3 inflammasome through targeting CD1d. WT mice were subjected to DSS-induced colitis. The protein expression of CD1d in colonic macrophages (A ) was detected by Western Blot. Data are presented as mean ± SD. **P < 0.01 vs. Normal group;#P < 0.05, ##P < 0.01 vs. DSS group. CD1d-/- mice and WT mice were intraperitoneally injected with AB4 (15mg/kg) for 7 days, and then given 3.0% DSS in drinking water for 7 days, during which AB4 (15mg/kg) was continuously injected intraperitoneally (n=8 per group). At the end of the experiment, the mice were sacrificed, and the colons were collected. (B ) The body weight change, (C ) the disease activity index (DAI), and (D ) the colon length was measured. (E ) H&E staining analysis of histopathological changes (left) and semi-quantitative scoring of histopathology (right) and the images was taken at 200x magnification (scale bar: 50μm). (F ) The infiltration of F4/80+ macrophages in colonic tissue was determined by using an immunofluorescence assay, and the images were taken at 200x magnification (scale bar: 50μm). Data are presented as mean ± SD. *P < 0.05CD1d-/- +DSS vs. WT+DSS;##P < 0.01CD1d-/- +DSS+ AB4 vs. WT+DSS+ AB4 group. (G ) The protein of the indicated molecule was detected by Western Blot. (H ) The production of IL-1β and IL-18 in colons was analyzed by ELISA. Data are presented as mean ± SD.##P < 0.01CD1d-/- +DSS+ AB4 vs. WT+DSS+ AB4 group.