DSS-induced colitis and design of drug treatment
C57BL/6 mice were fed with 3.0% (w/v ) DSS in drinking water for 7 days to induce acute colitis. In order to explore the impact of AB4 on colitis, the mice were randomly divided into 5 groups (n =6 in each group): Normal group, DSS group, 5-ASA (200 mg/kg) group, AB4 pretreatment group (5 mg/kg), and AB4 treatment group (5 mg/kg). To further confirm the protective effect of AB4, the mice were randomly divided into 6 groups (n =8 in each group): Normal group, DSS group, AB4 (5, 10, and 15mg/kg) groups, and AB4 (15mg/kg) alone group.
To further confirmed that the protective effect of AB4 in DSS-induced colitis is dependent on the intervention of NLRP3 inflammasome, WT and NLRP3-/- mice were randomly divided into 6 groups (n=8 in each group): WT normal group, WT+DSS group, WT+DSS+AB4 (15mg/kg) group, NLRP3-/- normal group, NLRP3-/-+DSS group, NLRP3-/-+DSS+AB4 (15mg/kg) group.
To investigate whether the protective effect of AB4 against colitis depends on the CD1d signaling pathway, the WT and CD1d-/- mice were randomly divided into 6 groups (n=8 in each group): WT normal group; WT+DSS group; WT+DSS+AB4 (15mg/kg) group; CD1d-/- normal group; CD1d-/-+DSS group; CD1d-/-+DSS+AB4 (15mg/kg) group. Observe and record the changes in body weight, blood in the stool, and diarrhea every day, and use a complete system to calculate the DAI score(Cui et al., 2020; Lv et al., 2021).