Figure 4. NLRP3 is critical for protection against DSS-induced injury by AB4. NLRP3-/- mice and WT mice were intraperitoneally injected with AB4 (15mg/kg) for 7 days, and then given 3.0% DSS in drinking water for 7 days, during which AB4 (15mg/kg) was continuously injected intraperitoneally (n=8 per group). At the end of the experiment, the mice were sacrificed, and the colons were collected. (A ) The body weight change, (B ) the disease activity index (DAI), and (C ) the colon length was measured. (D ) H&E staining analysis of histopathological changes (left) and semi-quantitative scoring of histopathology (right) and the images was taken at 200x magnification (scale bar: 50μm). Data are presented as mean ± SD. **P < 0.01 NLRP3-/-+DSSvs. WT+DSS; ns, not significantly different NLRP3-/-+DSS+AB4 vs.NLRP3-/-+DSS.