Animals
C57BL/6 mice (male, 20-22g, 6-8 weeks) were purchased from Dalian
Medical University (Dalian, China). NLRP3-deficient
(NLRP3-/-; #0017970) mice (male, 20-22g, 6-8 weeks)
and macrophage-specific CD1d deficient (CD1d-/-;
#008881) mice (male, 20-22g, 6-8 weeks) were obtained from the Third
Military Medical University (Department of Immunology, Chongqing,
China). All mice were housed at a temperature of 20-25℃ and a relative
humidity of 55%-65%, and free diet and water.
All animal experiments were approved
by the Animal Welfare and Ethics Committee of Dalian University (no.
SCAV-EXPANIM).