3.1 Genome sequencing and assembly
The genome assembly was 1.48 Gb in length, comprising 1,516 contigs with a contig N50 of 7.715 Mb (Tables S3-S4). Over 99.99% of raw reads were retained after quality trimming. The genome had 97.18% complete BUSCOs [Single copy: 96.4%; Duplicated: 0.7%], 0.5% fragmented BUSCOs and 2.4% missing BUSCOs. A total of 12,877 predicted genes were used as evidence for genome annotation from the global transcriptome, which had 94.9% complete BUSCOs [Single copy: 15.0%; Duplicated: 79.9%], 1.3% fragmented BUSCOs and 3.8% missing BUSCOs. After annotation, 30,347 genes were predicted, with an annotation completeness of 82.8% BUSCOs [Single copy: 82.1%; Duplicated: 0.7%], 7.7% fragmented BUSCOs and 9.5% missing BUSCOs. The mitochondrial genome was 16,867 bp in length and was identified on a single contig (Figure S4).