3. Conclusions
In this study, a novel
automated
robot-driven
PEC biosensing platform, integrating a CRISPR/Cas12a system and PEC
analysis, facilitated rapid, ultrasensitive, highly specific detection
of biological loads in sewage. By harnessing the remarkable conductivity
and extensive specific surface area offered by vertical graphene, the
excellent photoelectric characteristics of CdTe/ZnS QDs, and the high
specificity and efficient trans-cleavage activity of the Cas12a–Cas
system, the developed PEC biosensor exhibited
outstanding analytical
capabilities. The biosensor demonstrated an extensive linear range
spanning from 1.0 aM to 1.0 nM, a remarkable lower detection limit of
2.93×102 zM, and exceptional selectivity at the level
of single-base mutation recognition. Furthermore,
the
Shennong-1 robot was employed to
automate sewage sampling, enrichment, concentration, nucleic acid
extraction, and reverse transcription,
expediting the analysis of
Omicron BA.5 in sewage.
This innovative
robot- driven photoelectrochemical
biosensing platform promises swift, ultrasensitive, and highly precise
detection of Omicron BA.5, making it an excellent candidate for future
applications of sewage monitoring for biological loads.