4.1. Materials and reagents
K3Fe(CN)6,
K4Fe(CN)6, KCl,
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), andN -hydroxysuccinimide (NHS) were purchased from Aladdin
Biotechnology Co., Ltd. (Shanghai, China). Carboxylated CdTe/ZnS QDs was
purchased from Ruixi Biotechnology Co., Ltd. (Xi’an, China).
Phosphate-buffered saline (PBS) buffer (0.1 M, pH 7.4), and
2-(N -morpholino)ethanesulfonic acid (MES) buffer (0.1 M, pH 6.0)
were purchased from Phygene Biotechnology Co., Ltd. (Fuzhou, China).
Cas12a, 10× Cas12a Reaction Buffer and fluorescein amidite –
single-stranded DNA (FAM-ssDNA) reporter were purchased from EZassay
Biotechnology Co., Ltd. (Shenzhen, China). A nucleic acid extraction kit
(Magnetic Beads Method) was purchased from Zhongyuan Biotechnology Co.,
Ltd. (Chongqing, China). A PrimeScript™ RT reagent kit was purchased
from TaKaRa Biotechnology Co., Ltd. (Dalian, China).
Tris(2-carboxyethyl)phosphine hydrochloride (TCEP),
ethylenediaminetetraacetic acid (EDTA), and 6-mercaptohexanol (MCH) were
purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). The
construction of plasmids carrying the designed dsDNA template and the
synthesis of crRNA, ssDNAs 1, 2, and 3 (Table S1) were performed by
Sangon Biotech Co., Ltd. (Shanghai, China).