Experimental Design
Either iDREADDs or a green fluorescent protein (GFP) expressing
adeno-associated virus (control) was injected into the dorsal subiculum
in two separate groups of rats. Shortly before each behavioural test,
both groups received intracerebral infusions targeted at retrosplenial
cortex. For some sessions clozapine was infused, on other sessions it
was saline. Animals were then tested on reinforced alternation in a
T-maze, using five successive variants that differently taxed the use of
available cues. The experiment was repeated with two separate cohorts of
rats, both treated in the same ways (Figure 1).