Figure 3. Schematic representation of retrosplenial cannula placement for each experimental animal. Panel A shows coronal sections (cresyl violet) with cannulation sites in the anterior (left) and posterior (right) retrosplenial cortex. Panel B (below) is a schematic representation of cannula placements adapted from the Paxinos and Watson rat atlas (2004) for each animal in both the anterior (left) and posterior (right) portions of the retrosplenial cortex. Squares denote iDREADDs animals and triangles GFP-controls. In three iDREADDs and 1 GFP-control animal, the cannulas also affected the most posterior portions of the cingulate cortex. The same implantation coordinates were used for all animals, producing considerable overlap of cannula placements. The numbers represent the approximate distance from bregma in mm. All scale bars are 150µm. Abbreviations: Cg1/2, anterior cingulate cortex; M2, secondary motor cortex, RSD, dysgranular retrosplenial cortex; RSGc, granular retrosplenial cortex, area c; RSGb, granular retrosplenial cortex, area b; RSGa, granular retrosplenial cortex, area a; V2, secondary visual area.