Experimental Design
Either iDREADDs or a green fluorescent protein (GFP) expressing adeno-associated virus (control) was injected into the dorsal subiculum in two separate groups of rats. Shortly before each behavioural test, both groups received intracerebral infusions targeted at retrosplenial cortex. For some sessions clozapine was infused, on other sessions it was saline. Animals were then tested on reinforced alternation in a T-maze, using five successive variants that differently taxed the use of available cues. The experiment was repeated with two separate cohorts of rats, both treated in the same ways (Figure 1).