Figure
3. Comparison of the gut microbiome between wild and laboratory-raisedAstyanax mexicanus surface fish and cavefish morphotypes.
(A) Comparison of the number of species and their distributions in
intestinal microbiome of A. mexicanus surface and cavefish
populations as estimated by Shannon Index. Asterisks indicate
significance based on one-way ANOVA with Tukey’s post hoc test
(*p<0.05, **p<0.005).
(B) Variation in intestinal microbiome composition between A.
mexicanus surface fish and cavefish in the wild and lab. Distances
determined by Bray-Curtis. Ellipses estimate covariance and the centroid
of each cluster. PCoA used as the ordination method.
(C) Venn diagram showing shared number of ASVs between wild and
laboratory-raised surface fish and Pachón cavefish.
(D) Dendrogram of interspecific relatedness of the intestinal microbiome
of laboratory-raised A. mexicanus. ASV tables were collapsed by
the indicated population and Bray-Curtis distances were calculated for
each representative microbiota profile. The resulting Bray-Curtis
distance matrices were UPGMA clustered to produce the dendrogram.