Figure 4. Low abundance of Fusobacteriota in Astyanax mexicanus Pachón cavefish can be detected in individual fish over time using fecal samples.
  1. Bar graph showing average proportional abundance of Fusobacteriota ASVs in 16s rRNA sequencing of intestinal contents of A. mexicanus surface fish and cavefish raised in the laboratory (n = 35).
  2. Bar graph showing proportional abundance of Fusobacteriota ASVs in 16s rRNA sequencing in pooled fecal samples from A. mexicanussurface fish and cavefish morphotypes raised in the laboratory (n=4).
  3. Quantification of Fusobacteriota 16s rRNA and Eubacteria 16s rRNA in fecal samples from individual three-year-old siblings of the surface fish and Pachón cavefish used for intestinal microbiome sequencing. Greater quantification cycle indicates lower abundance. Eubacteria is not different between samples indicating the total amount of bacterial DNA has been properly controlled.
  4. Quantification of Fusobacteriota 16s rRNA and Eubacteria 16s rRNA in fecal samples collected on three different days from individual three-year-old siblings of surface fish and Pachón cavefish used for microbiome sequencing.