Figure
4. Low abundance of Fusobacteriota in Astyanax mexicanus Pachón
cavefish can be detected in individual fish over time using fecal
samples.
- Bar graph showing average proportional abundance of Fusobacteriota
ASVs in 16s rRNA sequencing of intestinal contents of A.
mexicanus surface fish and cavefish raised in the laboratory (n =
35).
- Bar graph showing proportional abundance of Fusobacteriota ASVs in 16s
rRNA sequencing in pooled fecal samples from A. mexicanussurface fish and cavefish morphotypes raised in the laboratory (n=4).
- Quantification of Fusobacteriota 16s rRNA and Eubacteria 16s rRNA in
fecal samples from individual three-year-old siblings of the surface
fish and Pachón cavefish used for intestinal microbiome sequencing.
Greater quantification cycle indicates lower abundance. Eubacteria is
not different between samples indicating the total amount of bacterial
DNA has been properly controlled.
- Quantification of Fusobacteriota 16s rRNA and Eubacteria 16s rRNA in
fecal samples collected on three different days from individual
three-year-old siblings of surface fish and Pachón cavefish used for
microbiome sequencing.