Figure 3. Comparison of the gut microbiome between wild and laboratory-raisedAstyanax mexicanus surface fish and cavefish morphotypes.
(A) Comparison of the number of species and their distributions in intestinal microbiome of A. mexicanus surface and cavefish populations as estimated by Shannon Index. Asterisks indicate significance based on one-way ANOVA with Tukey’s post hoc test (*p<0.05, **p<0.005). (B) Variation in intestinal microbiome composition between A. mexicanus surface fish and cavefish in the wild and lab. Distances determined by Bray-Curtis. Ellipses estimate covariance and the centroid of each cluster. PCoA used as the ordination method. (C) Venn diagram showing shared number of ASVs between wild and laboratory-raised surface fish and Pachón cavefish. (D) Dendrogram of interspecific relatedness of the intestinal microbiome of laboratory-raised A. mexicanus. ASV tables were collapsed by the indicated population and Bray-Curtis distances were calculated for each representative microbiota profile. The resulting Bray-Curtis distance matrices were UPGMA clustered to produce the dendrogram.