Figure Legends
Figure 1 – Serum cytokine concentrations in
allergen-sensitized subjects vs. controls. IL-26 (A) and IL-17A (B)
serum concentrations were measured by ELISA in both
non-allergen-sensitized and allergen-sensitized study subjects (n = 17
and n = 60, respectively). Statistical significance was assessed using
the Mann-Whitney test. Error bars represent mean ± SEM. Dashed horizonal
line represents the lower limit of detection of the ELISA assay.
Figure 2 – Serum cytokine concentrations in subjects with and
without specific allergic manifestations. Serum concentrations of IL-26
and IL-17A were measured by ELISA in both non-allergen-sensitized and
allergen-sensitized study subjects (n = 17 and n = 60, respectively).
Comparisons were made in subjects with or without asthma (A-B), current
or prior eczema (C-D), allergic rhinitis (E-F), and a history of one or
more food allergies (G-H). Statistical significance was assessed using
the Kruskal-Wallis test for multiple comparisons. Error bars represent
mean ± SEM. Dashed horizonal line represents the lower limit of
detection of the ELISA assay.
Figure 3 – Serum IL-26 concentrations correlate with serum
IL-17A in allergen-sensitized subjects. Serum concentrations of IL-26
and IL-17A were measured by ELISA in allergen-sensitized subjects.
Concentrations of each cytokine were plotted relative to each other for
(A) all allergen-sensitized subjects (n = 60) or (B) limited to only
allergen-sensitized subjects with asthma (n = 51). Statistical
significance was assessed using the Spearman correlation coefficient.
Figure 4 – Serum IL-26 concentrations correlate with Asthma
Control Test (ACT) Score in allergen-sensitized subjects. Serum
concentrations of IL-26 were measured by ELISA in allergen-sensitized
subjects with asthma (n = 51) and plotted relative to ACT scores.
Statistical significance was assessed using the Spearman correlation
coefficient.
Figure 5 – Increasing serum IL-26 concentrations correlate
with surrogate markers of asthma severity in allergen-sensitized
subjects. Serum concentrations of IL-26 were measured by ELISA in
allergen-sensitized subjects with asthma (n = 51). (A) IL-26
concentrations were plotted relative to each subject’s daily inhaled
corticosteroid dose. Statistical significance was assessed using the
Spearman correlation coefficient. (B) IL-26 concentrations were compared
between subjects who had no prior hospitalizations vs. one or more
hospitalizations for asthma in the 12 months preceding study enrolment.
(C) IL-26 concentrations were compared between subjects who had received
no oral steroid vs. those receiving one or more courses of oral steroids
in the 12 months preceding study enrolment. (B-C) Statistical
significance was assessed using the Mann-Whitney test. Error bars
represent mean ± SEM. Dashed horizonal line represents the lower limit
of detection of the ELISA assay.