Experimental approach
Co-immunoprecipitation was for STIM1-TRPC1 association analysis. Western
blotting and Real-time quantitative reverse transcription PCR (QPCR)
were for EMT analysis. Calcium imaging, flow cytometry, and luciferase
report assay were for analyzing calcium signaling. ELISA,
histomorphology, and PQ-poisoned mice or cynomolgus model were for
evaluating the efficacy of lysine in treating PF. Retrospective analysis
was for analyzing the correlation between the severity of PQ poisoning
and blood calcium levels.