2.2 Mice
Six-week-old male C57BL/6 mice were randomly and evenly divided into
groups as indicated in specific experiments. The mice were
intraperitoneally injected
with PQ (50 mg/kg, Sigma-Aldrich, 36541) for one time, whereas vehicle
control, SKF (10 mg/kg, MedChemExpress, HY-100001) or lysine (10 mg/kg,
Sangon Biotech, A602769) was
intraperitoneally injected
every day for 3 days, or as indicated. The weight, daily food and water
uptake of the treated mice were recorded every day. Mice were sacrificed
on day 4 or as indicated, and lung lobes were removed, fixed in 4%
paraformaldehyde, dehydrated, and subjected to paraffin embedding. Lung
microsections (5 µm) were stained with hematoxylin & eosin (H&E) and
Masson’s trichrome to visualize fibrotic lesions. Cheek blood samples
were prepared and the serum was isolated by centrifugation with 2000 rpm
for 5 min. The serum was monitored in Servicebio (Wuhan, China) for
blood levels of calcium, ALT, AST, and creatinine. All the animal
studies were approved by the Ethics Committee of Shanghai General
Hospital.