Experimental approach
Co-immunoprecipitation was for STIM1-TRPC1 association analysis. Western blotting and Real-time quantitative reverse transcription PCR (QPCR) were for EMT analysis. Calcium imaging, flow cytometry, and luciferase report assay were for analyzing calcium signaling. ELISA, histomorphology, and PQ-poisoned mice or cynomolgus model were for evaluating the efficacy of lysine in treating PF. Retrospective analysis was for analyzing the correlation between the severity of PQ poisoning and blood calcium levels.