2.6 Quantitative RT-PCR
A549 cells were treated with PQ in a dose dependent manner, as
indicated, with or without 5 mM lysine, for 24h. The cells were lysed in
TRIZOL (Vazyme Biotech Co, R701-02) and total RNA was extracted and
reverse transcribed into cDNA by HiScript III RT SuperMix kit (Vazyme
Biotech Co, R323). The mRNA expression of E-cadherin and Vimentin was
determined by semi-quantification with ChamQ SYBR Color qPCR Master Mix
(Vazyme Biotech Co, R323, Q411-02) in QuantStudio 7 Flex. Data were
normalized to Actin and calculated by 2[− (Ct target
gene−Ct Actin)]. The sequence of the PCR primers was previously
reported(Yang et al., 2022).