2.1 Cell Culture
A549 cells were cultured in Ham’s F-12K Medium (BasalMedia, L450KJ)
supplemented with 10% fetal bovine serum (GIBCO, 42Q1095K), penicillin
(100 IU/ml, Genom, GNM15140) and streptomycin (100 µg/ml, Genom,
GNM15140). MLE-12 cells were cultured in Dulbecco’s Modified Eagle
Medium/Nutrient Mixture F-12 (DMEM/F12, AWcell-0005) supplemented with
2% fetal bovine serum (GIBCO, 42Q1095K), 1% GlutaMAX-1 (AWcell-0900),
10 mM HEPES buffer solution (GIBCO, 15630-080), 1% ITS (AWcell-4507),
10 nM hydrocortisone (AWcell-8450), 10 nM β-estradiol (AWcell-8140),
penicillin (100 IU/ml, Genom, GNM15140) and streptomycin (100 μg/ml,
Genom, GNM15140). These cell lines were grown at 37°C in a 5% carbon
dioxide incubator and were passaged following trypsinization.