2.1 Cell Culture
A549 cells were cultured in Ham’s F-12K Medium (BasalMedia, L450KJ) supplemented with 10% fetal bovine serum (GIBCO, 42Q1095K), penicillin (100 IU/ml, Genom, GNM15140) and streptomycin (100 µg/ml, Genom, GNM15140). MLE-12 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12, AWcell-0005) supplemented with 2% fetal bovine serum (GIBCO, 42Q1095K), 1% GlutaMAX-1 (AWcell-0900), 10 mM HEPES buffer solution (GIBCO, 15630-080), 1% ITS (AWcell-4507), 10 nM hydrocortisone (AWcell-8450), 10 nM β-estradiol (AWcell-8140), penicillin (100 IU/ml, Genom, GNM15140) and streptomycin (100 μg/ml, Genom, GNM15140). These cell lines were grown at 37°C in a 5% carbon dioxide incubator and were passaged following trypsinization.