2.7 Western blot
Cells were harvested and the protein lysates were separated by 10%
SDS-PAGE gels and transferred onto a nitrocellulose filter membrane (NC)
membrane (Pall,66485), blocked with 5% non-fat milk in phosphate
buffered solution with 0.1% Tween 20 (PBST). Primary antibodies were
incubated at 4 ℃ overnight, including Anti-STIM1 (Cell Signaling
Technology, 5668, 1:1000), Anti-ORAI1 (Santa Cruz, sc-377281, 1:500),
Anti-TRPC1 (Proteintech, 19482, 1:1000), Anti-E-cadherin (Cell Signaling
Technology, 3195S, 1:1000), Anti-Vimentin (Cell Signaling Technology,
5741S, 1:1000), and Anti-GAPDH (Proteintech, 60004-1-1g, 1:10000).