2.3 PQ-poisoned cynomolgus model
The PQ-poisoned cynomolgus model was performed at Medicilon Inc. (Shanghai, China). Briefly, five-year-old male cynomolgus monkeys were orally administrated with 12.5 mg/kg PQ once, together with vehicle or 40 mg/kg lysine by intravenous injection every day for three days. Blood was collected by femoral venipuncture as indicated. The population of immune cells was measured by laboratory blood tests and the blood calcium levels were monitored by serum biochemistry. For CRP analysis, serum was prepared by the centrifugation of collected blood with 3500 rpm for 10 min at room temperature. The prepared serum was then subjected to CRP ELISA analysis according to the manufacturer’s instructions.