Figure 2. S. aureus intracellular invasion, localization and replication within LAD2 mast cells.
Intracellular survival of S. aureus co-cultured with LAD2 mast cells. A control S. aureus strain (blue) was compared with a CRSwNP S. aureus strain (yellow) at time points of 3, 6, 9 hours (n=9, mean + SEM,*p≤0.05, ** p≤0.01). Extracellular bacteria was eradicated at 6 hours for the control (blue/grey checkboard) and CRSwNP isolates (yellow/grey checkboard) using lysostaphin and the culture continued to 24 hours (n=6, mean + SEM, *p≤0.05).