4.2 Effect of N addition on the structure and diversity of AM
fungi community
Different N addition content and local soil conditions affect plants and
soil microorganisms (Cao et al., 2020; Zeng et al., 2021). In this
study, both high-throughput sequencing and traditional spore
morphological identification were used to investigate the community
structure and diversity of woodland AM fungi. Morphological
identification has inconsistencies, limitations, and chances, but can be
improved by means such as increasing the sample size.
High-throughput sequencing
technology has been widely used by researchers, but stills has some
limitations. Due to the variety of microorganisms, the current database
cannot cover all the microorganisms or lacks accurate species
information, resulting in the omission of some low-abundance species, so
we combine the two methods to accurately reflect the diversity and
structural characteristics of AM fungi (Chaudhary, Nolimal,
Sosa-Hernandez, Egan , & Kastens, 2020). Both high-throughput
sequencing results and morphological identification results showed that
N addition altered AM fungi community composition and reduced AM fungal
alpha diversity.
It is clear from the RDA analysis (Fig. 5) that pH significantly
affected the community composition of AM fungi in Korean pine
plantation, and there was research reported that soil pH was one of the
most important abiotic factors affecting the ecological distribution of
AM fungi (Davison et al., 2021). The abundance of Glomusincreased gradually (Fig. 4) with increasing N addition concentration
and was significantly higher in the HN treatment, the main reason for
this phenomenon being that Glomus showed a better ecological
advantage in acidic soils. In the experiments of (Cao et al., 2020),
short-term HN treatment (80 kg N ha-1year-1) also reduced fungal diversity and changed
community composition. It is believed that this phenomenon is caused by
the change of P elements in the soil due to N addition, which causes
plants to select AM fungi with high phosphorus absorption capacity