2.6 Data analyses
Data are the mean ± SEM. SBCs was measured at 15-min intervals immediately before (control) and after drug application. The response to H2O2 was evaluated as the percent increase in the area under the curve (AUC, an integrative of the amplitude, baseline tension, and frequency of phasic contractions) as well as the net increase in baseline tension (Δg) from the control. Δg was normalized with the wet weight of strips (Δg/g). Concentration–response curves were fitted with the Hill equation: Percent enhancement = (MAXenhancement/[drug concentration + EC50])n where MAXenhancement is maximal % enhancement, drug concentration is the agonist concentration, EC50 is the half-maximal effective concentration, and n is the Hill coefficient. Statistical significance was tested with the one-sample t -test or paired t -test (two-tailed). Statistical analyses were undertaken with Excel™ (Microsoft, Redmond, WA, USA) and Prism 8 (GraphPad, La Jolla, CA, USA). p < 0.05 was considered significant.