2.6 Data analyses
Data are the mean ± SEM. SBCs was measured at 15-min intervals
immediately before (control) and after drug application. The response to
H2O2 was evaluated as the percent
increase in the area under the curve (AUC, an integrative of the
amplitude, baseline tension, and frequency of phasic contractions) as
well as the net increase in baseline tension (Δg) from the control. Δg
was normalized with the wet weight of strips (Δg/g).
Concentration–response curves were fitted with the Hill equation:
Percent enhancement = (MAXenhancement/[drug
concentration + EC50])n where
MAXenhancement is maximal % enhancement, drug
concentration is the agonist concentration, EC50 is the
half-maximal effective concentration, and n is the Hill coefficient.
Statistical significance was tested with the one-sample t -test or
paired t -test (two-tailed). Statistical analyses were undertaken
with Excel™ (Microsoft, Redmond, WA, USA) and Prism 8 (GraphPad, La
Jolla, CA, USA). p < 0.05 was considered significant.